PMID- 38508727
OWN - NLM
STAT- MEDLINE
DCOM- 20240710
LR  - 20240806
IS  - 1881-7122 (Electronic)
IS  - 1341-1357 (Print)
IS  - 0007-5124 (Linking)
VI  - 73
IP  - 3
DP  - 2024 Jul 9
TI  - Transient receptor potential vanilloid 1 interacts with Toll-like receptor 4 
      (TLR4)/cluster of differentiation 14 (CD14) signaling pathway in 
      lipopolysaccharide-mediated inflammation in macrophages.
PG  - 336-346
LID - 10.1538/expanim.23-0148 [doi]
AB  - Transient receptor potential vanilloid 1 (TRPV1), a ligand-gated cation channel, 
      is a receptor for vanilloids on sensory neurons and is also activated by 
      capsaicin, heat, protons, arachidonic acid metabolites, and inflammatory 
      mediators on neuronal or non-neuronal cells. However, the role of the TRPV1 
      receptor in pro-inflammatory cytokine secretion and its potential regulatory 
      mechanisms in lipopolysaccharide (LPS)-induced inflammation has yet to be 
      entirely understood. To investigate the role and regulatory mechanism of the 
      TRPV1 receptor in regulating LPS-induced inflammatory responses, bone 
      marrow-derived macrophages (BMDMs) harvested from wild-type (WT) and TRPV1 
      deficient (Trpv1(-/-)) mice were used as the cell model. In WT BMDMs, LPS induced 
      an increase in the levels of tumor necrosis factor-alpha, IL-1beta, inducible nitric 
      oxide synthase, and nitric oxide, which were attenuated in Trpv1(-/-) BMDMs. 
      Additionally, the phosphorylation of inhibitor of nuclear factor kappa-Balpha and 
      mitogen-activated protein kinases, as well as the translocation of nuclear factor 
      kappa-B and activator protein 1, were all decreased in LPS-treated Trpv1(-/-) 
      BMDMs. Immunoprecipitation assay revealed that LPS treatment increased the 
      formation of TRPV1-Toll-like receptor 4 (TLR4)-cluster of differentiation 14 
      (CD14) complex in WT BMDMs. Genetic deletion of TRPV1 in BMDMs impaired the 
      LPS-triggered immune-complex formation of TLR4, myeloid differentiation protein 
      88, and interleukin-1 receptor-associated kinase, all of which are essential 
      regulators in LPS-induced activation of the TLR4 signaling pathway. Moreover, 
      genetic deletion of TRPV1 prevented the LPS-induced lethality and 
      pro-inflammatory production in mice. In conclusion, the TRPV1 receptor may 
      positively regulate the LPS-mediated inflammatory responses in macrophages by 
      increasing the interaction with the TLR4-CD14 complex and activating the 
      downstream signaling cascade.
FAU - Hsu, Julia Chu-Ning
AU  - Hsu JC
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, National Chung 
      Hsing University, 145, Xingda Road, South District, Taichung 402202, Taiwan.
FAU - Tseng, Hsu-Wen
AU  - Tseng HW
AD  - Department of Physiology, School of Medicine, National Yang-Ming University, 155, 
      Sec. 2, Linong Street, Taipei 112304, Taiwan.
FAU - Chen, Chia-Hui
AU  - Chen CH
AD  - Graduate Institute and Department of Physiology, College of Medicine, National 
      Taiwan University, 1, Sec. 1, Jen-Ai Road, Taipei 100233, Taiwan.
FAU - Lee, Tzong-Shyuan
AU  - Lee TS
AD  - Graduate Institute and Department of Physiology, College of Medicine, National 
      Taiwan University, 1, Sec. 1, Jen-Ai Road, Taipei 100233, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20240322
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TRPV1 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Cd14 protein, mouse)
SB  - IM
MH  - Animals
MH  - *TRPV Cation Channels/metabolism/genetics/physiology
MH  - *Toll-Like Receptor 4/metabolism/genetics/physiology
MH  - *Lipopolysaccharides
MH  - *Signal Transduction
MH  - *Macrophages/metabolism
MH  - *Lipopolysaccharide Receptors/metabolism/genetics
MH  - *Inflammation/metabolism/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Male
PMC - PMC11254490
OTO - NOTNLM
OT  - Toll-like receptor 4 (TLR4)
OT  - inflammation
OT  - lipopolysaccharide (LPS)
OT  - macrophage
OT  - transient receptor potential vanilloid 1 (TRPV1)
COIS- The authors declare no conflict of interest.
EDAT- 2024/03/21 00:43
MHDA- 2024/07/11 00:42
PMCR- 2024/01/01
CRDT- 2024/03/20 21:43
PHST- 2024/07/11 00:42 [medline]
PHST- 2024/03/21 00:43 [pubmed]
PHST- 2024/03/20 21:43 [entrez]
PHST- 2024/01/01 00:00 [pmc-release]
AID - 23-0148 [pii]
AID - 10.1538/expanim.23-0148 [doi]
PST - ppublish
SO  - Exp Anim. 2024 Jul 9;73(3):336-346. doi: 10.1538/expanim.23-0148. Epub 2024 Mar 
      22.