PMID- 38508929
OWN - NLM
STAT- MEDLINE
DCOM- 20240416
LR  - 20240416
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 42
IP  - 11
DP  - 2024 Apr 19
TI  - Subolesin knockdown in tick cells provides insights into vaccine protective 
      mechanisms.
PG  - 2801-2809
LID - S0264-410X(24)00278-0 [pii]
LID - 10.1016/j.vaccine.2024.03.006 [doi]
AB  - Ticks as obligate blood-feeding arthropod vectors of pathogenic viruses, 
      bacteria, protozoa and helminths associated with prevalent tick-borne diseases 
      (TBDs) worldwide. These arthropods constitute the second vector after mosquitoes 
      that transmit pathogens to humans and the first vector in domestic animals. 
      Vaccines constitute the safest and more effective approach to control tick 
      infestations and TBDs, but research is needed to identify new antigens and 
      improve vaccine formulations. The tick protein Subolesin (Sub) is a well-known 
      vaccine protective antigen with a highly conserved sequence at both gene and 
      protein levels in the Ixodidae and among arthropods and vertebrates. In this 
      study, transcriptomics and proteomics analyses were conducted together with graph 
      theory data analysis in wild type and Sub knockdown (KD) tick ISE6 cells in order 
      to identify and characterize the functional implications of Sub in tick cells. 
      The results support a key role for Sub in the regulation of gene expression in 
      ticks and the relevance of this antigen in vaccine development against ticks and 
      TBDs. Proteins with differential representation in response to Sub KD provide 
      insights into vaccine protective mechanisms and candidate tick protective 
      antigens.
CI  - Copyright (c) 2024 Elsevier Ltd. All rights reserved.
FAU - Artigas-Jeronimo, Sara
AU  - Artigas-Jeronimo S
AD  - Biochemistry Section, Faculty of Science and Chemical Technologies, University of 
      Castilla- La Mancha, 13071 Ciudad Real, Spain. Electronic address: 
      sara.artigas@uclm.es.
FAU - Villar, Margarita
AU  - Villar M
AD  - Biochemistry Section, Faculty of Science and Chemical Technologies, University of 
      Castilla- La Mancha, 13071 Ciudad Real, Spain; SaBio, Instituto de Investigacion 
      en Recursos Cinegeticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo 12, 13005 Ciudad 
      Real, Spain.
FAU - Estrada-Pena, Agustin
AU  - Estrada-Pena A
AD  - Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
FAU - Alberdi, Pilar
AU  - Alberdi P
AD  - SaBio, Instituto de Investigacion en Recursos Cinegeticos IREC-CSIC-UCLM-JCCM, 
      Ronda de Toledo 12, 13005 Ciudad Real, Spain.
FAU - de la Fuente, Jose
AU  - de la Fuente J
AD  - SaBio, Instituto de Investigacion en Recursos Cinegeticos IREC-CSIC-UCLM-JCCM, 
      Ronda de Toledo 12, 13005 Ciudad Real, Spain; Center for Veterinary Health 
      Sciences, Department of Veterinary Pathobiology, Oklahoma State University, 
      Stillwater, OK 74078, USA. Electronic address: jose_delafuente@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20240319
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Vaccines)
RN  - 0 (Antigens)
RN  - 0 (Arthropod Proteins)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Ticks/microbiology
MH  - Mosquito Vectors
MH  - *Vaccines
MH  - Antigens
MH  - *Tick Infestations/prevention & control
MH  - Arthropod Proteins/genetics
MH  - *Tick-Borne Diseases/prevention & control
OTO - NOTNLM
OT  - Antigen
OT  - ISE6 cells
OT  - Omics
OT  - Regulome
OT  - Subolesin
OT  - Tick
OT  - Vaccine
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/03/21 00:44
MHDA- 2024/04/16 12:42
CRDT- 2024/03/20 22:59
PHST- 2023/08/07 00:00 [received]
PHST- 2023/12/19 00:00 [revised]
PHST- 2024/03/04 00:00 [accepted]
PHST- 2024/04/16 12:42 [medline]
PHST- 2024/03/21 00:44 [pubmed]
PHST- 2024/03/20 22:59 [entrez]
AID - S0264-410X(24)00278-0 [pii]
AID - 10.1016/j.vaccine.2024.03.006 [doi]
PST - ppublish
SO  - Vaccine. 2024 Apr 19;42(11):2801-2809. doi: 10.1016/j.vaccine.2024.03.006. Epub 
      2024 Mar 19.