PMID- 38509956
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240322
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 10
IP  - 6
DP  - 2024 Mar 30
TI  - The role of matrix metalloproteinase-2 and miR-196a2 in bronchial asthma 
      pathogenesis and diagnosis.
PG  - e27694
LID - 10.1016/j.heliyon.2024.e27694 [doi]
LID - e27694
AB  - BACKGROUND: Bronchial asthma is a persistent inflammatory respiratory condition 
      that restricts the passage of air and causes hyperresponsiveness. Chronic asthma 
      can be classified into three categories: mild, moderate, and severe. Remodeling 
      took place as the extracellular matrix accumulated in the walls of the airways. 
      Inflammation occurs as a result of the damage caused by matrix 
      metalloproteinase-2 (MMP-2) to basement membrane type IV collagen. The severity 
      of asthma may be associated with miR-196a2. The objective of our study was to 
      investigate the underlying mechanisms and clinical relevance of miR-196a2 and 
      MMP-2 serum levels in relation to the severity of asthma. METHODS: This study 
      recruited 85 controls and 95 asthmatics classified as mild, moderate, or severe. 
      Expression of miR-196a2 was measured by quantitative reverse transcriptase PCR. 
      Using the enzyme-linked immunosorbent assay (ELISA), MMP-2, IL-6, and total 
      immunoglobulin E (IgE) levels in the serum of asthmatics of various grades were 
      compared to a control group. MMP-2's diagnostic and prognostic potential was 
      determined using ROC curve analysis. This study also measured blood Eosinophils 
      and PFTs. We examined MMP-2's connections with IgE, blood Eosinophils, and PFTs. 
      RESULTS: The current investigation found that miR-196a2 expression was 
      significantly higher in the control group than in asthmatic patients as a whole. 
      The study found that severe asthmatics had higher MMP-2, IL-6, and IgE serum 
      levels than healthy controls. We identified the MMP-2 serum concentration cutoff 
      with great sensitivity and specificity. Significant relationships between MMP-2 
      serum level and miR-196a2 expression in the patient group with severe asthmatics 
      were found. The MMP-2, IL-6, and IgE serum levels were considerably higher in 
      mild, moderate, and severe asthmatics than controls. The miR-196a2 expression and 
      MMP-2 serum concentration correlated positively with IgE and blood eosinophils % 
      and negatively with all lung function tests in the asthmatic patient 
      group.Conclusion: the study revealed that the elevated miR-196a2 expression and 
      serum concentration of MMP-2, IL-6, and IgE associated with elevated blood 
      eosinophils % is associated with pathophysiology and degree of asthma severity. 
      The miR-196a2 expression and MMP-2 serum concentration have a promising 
      diagnostic and prognostic ability in bronchial asthma.
CI  - (c) 2024 The Authors.
FAU - Mohammed, Osama A
AU  - Mohammed OA
AD  - Department of Pharmacology, College of Medicine, University of Bisha, Bisha, 
      61922, Saudi Arabia.
FAU - Doghish, Ahmed S
AU  - Doghish AS
AD  - Department of Biochemistry, Badr University in Cairo (BUC), Badr City, Cairo, 
      11829, Egypt.
AD  - Department of Biochemistry and Molecular Biology, Al-Azhar University, Nasr City, 
      Cairo, 11231, Egypt.
FAU - Alamri, Mohannad Mohammad S
AU  - Alamri MMS
AD  - Department of Family and Community Medicine, College of Medicine, University of 
      Bisha, Bisha, 61922, Saudi Arabia.
FAU - Alharthi, Muffarah Hamid
AU  - Alharthi MH
AD  - Department of Family and Community Medicine, College of Medicine, University of 
      Bisha, Bisha, 61922, Saudi Arabia.
FAU - Alfaifi, Jaber
AU  - Alfaifi J
AD  - Department of Child Health, College of Medicine, University of Bisha, Bisha, 
      61922, Saudi Arabia.
FAU - Adam, Masoud I E
AU  - Adam MIE
AD  - Department of Medical Education and Internal Medicine, College of Medicine, 
      University of Bisha, Bisha, 61922, Saudi Arabia.
FAU - Alhalafi, Abdullah Hassan
AU  - Alhalafi AH
AD  - Department of Family and Community Medicine, College of Medicine, University of 
      Bisha, Bisha, 61922, Saudi Arabia.
FAU - AlQahtani, AbdulElah Al Jarallah
AU  - AlQahtani AAJ
AD  - Department of Internal Medicine, Division of Dermatology, College of Medicine, 
      University of Bisha, Bisha, 61922, Saudi Arabia.
FAU - Rezigalla, Assad Ali
AU  - Rezigalla AA
AD  - Department of Anatomy, College of Medicine, University of Bisha, Bisha, 61922, 
      Saudi Arabia.
FAU - Taura, Magaji Garba
AU  - Taura MG
AD  - Department of Anatomy, College of Medicine, University of Bisha, Bisha, 61922, 
      Saudi Arabia.
FAU - Isa, Adamu Imam
AU  - Isa AI
AD  - Department of Physiology, College of Medicine, University of Bisha, Bisha, 61922, 
      Saudi Arabia.
FAU - Binafif, Ahad Fuad
AU  - Binafif AF
AD  - Blood Transfusion Services Center, Health Support Services Center, Ministry of 
      Health, Riyadh, 11176, Saudi Arabia.
FAU - Attia, Mohammed A
AU  - Attia MA
AD  - Department of Clinical Pharmacology, Mansoura University, Mansoura, 35516, Egypt.
AD  - Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, 
      Riyadh, 11597, Saudi Arabia.
FAU - Elmorsy, Elsayed A
AU  - Elmorsy EA
AD  - Department of Clinical Pharmacology, Mansoura University, Mansoura, 35516, Egypt.
AD  - Department of Pharmacology and Therapeutics, College of Medicine, Qassim 
      University, Buraydah, 51452, Saudi Arabia.
FAU - Yousef, Ayman A
AU  - Yousef AA
AD  - Chest Department, Benha University Hospitals, Qaliubyia, Egypt.
FAU - Abdel-Reheim, Mustafa Ahmed
AU  - Abdel-Reheim MA
AD  - Department of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, 
      Shaqra, 11961, Saudi Arabia.
AD  - Department of Pharmacology and Toxicology, Beni-Suef University, Beni.Suef, 
      62521, Egypt.
FAU - Elkady, Mohamed A
AU  - Elkady MA
AD  - Department of Biochemistry and Molecular Biology, Al-Azhar University, Nasr City, 
      Cairo, 11231, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20240312
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10950666
OTO - NOTNLM
OT  - Asthma
OT  - Diagnosis
OT  - IL-6
OT  - Matrix metalloproteinase-2
OT  - Pathophysiology
OT  - miR-196a2
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2024/03/21 06:43
MHDA- 2024/03/21 06:44
PMCR- 2024/03/12
CRDT- 2024/03/21 03:57
PHST- 2023/11/04 00:00 [received]
PHST- 2024/03/04 00:00 [revised]
PHST- 2024/03/05 00:00 [accepted]
PHST- 2024/03/21 06:44 [medline]
PHST- 2024/03/21 06:43 [pubmed]
PHST- 2024/03/21 03:57 [entrez]
PHST- 2024/03/12 00:00 [pmc-release]
AID - S2405-8440(24)03725-3 [pii]
AID - e27694 [pii]
AID - 10.1016/j.heliyon.2024.e27694 [doi]
PST - epublish
SO  - Heliyon. 2024 Mar 12;10(6):e27694. doi: 10.1016/j.heliyon.2024.e27694. 
      eCollection 2024 Mar 30.