PMID- 38512724
OWN - NLM
STAT- MEDLINE
DCOM- 20240325
LR  - 20240325
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 12
DP  - 2024 Mar 21
TI  - Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules 
      promotes radial migration of developing cortical neurons.
LID - 10.7554/eLife.92342 [doi]
LID - RP92342
AB  - Radial neuronal migration is a key neurodevelopmental event for proper cortical 
      laminar organization. The multipolar-to-bipolar transition, a critical step in 
      establishing neuronal polarity during radial migration, occurs in the 
      subplate/intermediate zone (SP/IZ), a distinct region of the embryonic cerebral 
      cortex. It has been known that the extracellular matrix (ECM) molecules are 
      enriched in the SP/IZ. However, the molecular constitution and functions of the 
      ECM formed in this region remain poorly understood. Here, we identified neurocan 
      (NCAN) as a major chondroitin sulfate proteoglycan in the mouse SP/IZ. NCAN binds 
      to both radial glial-cell-derived tenascin-C (TNC) and hyaluronan (HA), a large 
      linear polysaccharide, forming a ternary complex of NCAN, TNC, and HA in the 
      SP/IZ. Developing cortical neurons make contact with the ternary complex during 
      migration. The enzymatic or genetic disruption of the ternary complex impairs 
      radial migration by suppressing the multipolar-to-bipolar transition. 
      Furthermore, both TNC and NCAN promoted the morphological maturation of cortical 
      neurons in vitro. The present results provide evidence for the cooperative role 
      of neuron- and radial glial-cell-derived ECM molecules in cortical development.
CI  - (c) 2023, Mubuchi et al.
FAU - Mubuchi, Ayumu
AU  - Mubuchi A
AD  - Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 
      Fuchu, Japan.
FAU - Takechi, Mina
AU  - Takechi M
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
FAU - Nishio, Shunsuke
AU  - Nishio S
AUID- ORCID: 0000-0003-3420-2578
AD  - Faculty of Food and Agricultural Sciences, Fukushima University, Fukushima, 
      Japan.
FAU - Matsuda, Tsukasa
AU  - Matsuda T
AD  - Faculty of Food and Agricultural Sciences, Fukushima University, Fukushima, 
      Japan.
FAU - Itoh, Yoshifumi
AU  - Itoh Y
AUID- ORCID: 0000-0002-2128-2823
AD  - Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom.
FAU - Sato, Chihiro
AU  - Sato C
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
AD  - Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan.
AD  - Institute for Glyco-core Research, Nagoya University, Nagoya, Japan.
FAU - Kitajima, Ken
AU  - Kitajima K
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
AD  - Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan.
AD  - Institute for Glyco-core Research, Nagoya University, Nagoya, Japan.
FAU - Kitagawa, Hiroshi
AU  - Kitagawa H
AUID- ORCID: 0000-0002-9307-7079
AD  - Laboratory of Biochemistry, Kobe Pharmaceutical University, Kobe, Japan.
FAU - Miyata, Shinji
AU  - Miyata S
AUID- ORCID: 0000-0001-6635-1645
AD  - Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 
      Fuchu, Japan.
LA  - eng
GR  - 15K21067/Ministry of Education, Culture, Sports, Science and Technology/
GR  - 18K06130/Ministry of Education, Culture, Sports, Science and Technology/
GR  - 21H05681/Ministry of Education, Culture, Sports, Science and Technology/
GR  - Lotte Shigemitsu Prize/Lotte Foundation/
PT  - Journal Article
DEP - 20240321
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 0 (Chondroitin Sulfate Proteoglycans)
SB  - IM
UOF - doi: 10.1101/2022.09.03.506497
UOF - doi: 10.7554/eLife.92342.1
UOF - doi: 10.7554/eLife.92342.2
MH  - Animals
MH  - Mice
MH  - *Neurons/physiology
MH  - *Extracellular Matrix/metabolism
MH  - Cerebral Cortex/metabolism
MH  - Cell Movement/physiology
MH  - Chondroitin Sulfate Proteoglycans/metabolism
PMC - PMC10957175
OTO - NOTNLM
OT  - biochemistry
OT  - chemical biology
OT  - cortical development
OT  - extracellular matrix
OT  - hyaluronic acid
OT  - mouse
OT  - neuroscience
OT  - proteoglycans
OT  - radial migration
OT  - tenascin
COIS- AM, MT, SN, TM, YI, CS, KK, HK, SM No competing interests declared
EDAT- 2024/03/21 18:45
MHDA- 2024/03/25 06:42
PMCR- 2024/03/21
CRDT- 2024/03/21 12:53
PHST- 2024/03/25 06:42 [medline]
PHST- 2024/03/21 18:45 [pubmed]
PHST- 2024/03/21 12:53 [entrez]
PHST- 2024/03/21 00:00 [pmc-release]
AID - 92342 [pii]
AID - 10.7554/eLife.92342 [doi]
PST - epublish
SO  - Elife. 2024 Mar 21;12:RP92342. doi: 10.7554/eLife.92342.