PMID- 38515909
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240323
IS  - 2772-6533 (Electronic)
IS  - 2772-6533 (Linking)
VI  - 1
DP  - 2022 Dec
TI  - Assessing the variability and the role of inflammatory cytokines and monocyte 
      chemoattractant protein-1 (MCP-1) in predicting stroke among hypertensives: A 
      case-control study.
PG  - 100086
LID - 10.1016/j.dialog.2022.100086 [doi]
LID - 100086
AB  - BACKGROUND: Atherosclerosis is a complex lipid-driven inflammatory disease in 
      which numerous cell types and inflammatory mediators are involved in the 
      progression of hypertension to stroke. Mediators' markers that could predict the 
      progression of hypertension to stroke are of research importance. We assessed the 
      predictive value of individual and combined cytokines and monocyte 
      chemoattractant protein-1 (MCP-1) among hypertensives with or without stroke. 
      METHODS: In a case-control study, we enrolled 63 cases with stroke and 
      hypertension (HPT-S), 59 stroke-free hypertensives (HPT), and 53 stroke free 
      normotensives as controls (CS). Sociodemographic data and blood samples were 
      collected for the estimation of Interleukin-10 (IL-10), IL-6, IL-8, IL-1beta and 
      monocyte chemoattractant protein-1 (MCP-1) using commercially available ELISA 
      kits from Biobase Biotech, Shanghai, China. The Receiver Operator Characteristics 
      (ROC) analysis was used to calculate diagnostic accuracy for cytokines in 
      predicting stroke among hypertensives. A combined bioscore model of IL-10 and 
      MCP-1 was generated to predict stroke among hypertensives. The multiple logistic 
      regression analysis was used to assess the chances of IL-10 and MCP-1 in 
      predicting stroke among hypertensives. Statistical analyses were performed using 
      R-language. RESULTS: The HPT-S group had significantly higher levels of MCP-1 and 
      IL-10 compared to the HPT and CS groups (p < 0.05). There was no significant 
      difference in IL-1beta, IL-8 and IL-6 amongst the three study groups. MCP-1 and 
      IL-10 were predictive of stroke occurrence among hypertensives and were used to 
      develop a bioscore model. An elevated MCP-1 and IL-10 with a bioscore 2 had a 
      predictive accuracy of 0.81, a sensitivity of 0.77 and specificity of 0.84. At a 
      bioscore of 1, the sensitivity and specificity for predicting stroke among 
      hypertensives was 97.0% and 61.0% respectively. In a binary logistic regression, 
      having a bioscore of 1 [aOR = 20.43, 95% CI (2.17-192.62), p = 0.008] or 2 [aOR = 
      26.00, 95% CI (2.92-231.31), p = 0.003] were significantly associated with stroke 
      occurrence among hypertensives. CONCLUSION: Higher levels of IL-10 with a 
      concomitant level of MCP-1 could serve as a good predictor of stroke among 
      hypertensives. Subsequently, MCP-1 may prove useful as a therapeutic target for 
      atherosclerosis in hypertensives. Combined bioscore of MCP-1 and IL-10 could 
      serve as a good predictor of stroke among hypertensives.
CI  - (c) 2022 The Authors.
FAU - Sakyi, Samuel Asamoah
AU  - Sakyi SA
AD  - Department of Molecular Medicine, School of Medicine and Dentistry, Kwame Nkrumah 
      University of Science and Technology, Kumasi, Ghana.
FAU - Opoku, Afia Serwaa
AU  - Opoku AS
AD  - Department of Molecular Medicine, School of Medicine and Dentistry, Kwame Nkrumah 
      University of Science and Technology, Kumasi, Ghana.
FAU - Amoani, Benjamin
AU  - Amoani B
AD  - Department of Biomedical Science, School of Allied Health Sciences, University of 
      Cape Coast, Cape Coast, Ghana.
FAU - Afranie, Bright Oppong
AU  - Afranie BO
AD  - Department of Molecular Medicine, School of Medicine and Dentistry, Kwame Nkrumah 
      University of Science and Technology, Kumasi, Ghana.
FAU - Kwarteng, Alexander
AU  - Kwarteng A
AD  - Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah 
      University of Science and Technology, Kumasi, Ghana.
FAU - Ephriam, Richard Dadzie
AU  - Ephriam RD
AD  - Department of Medical Laboratory Sciences, School of Allied Health Sciences, 
      College of Health and Allied Sciences, University of Cape Coast, Cape Coast, 
      Ghana.
FAU - Opoku, Stephen
AU  - Opoku S
AD  - Department of Molecular Medicine, School of Medicine and Dentistry, Kwame Nkrumah 
      University of Science and Technology, Kumasi, Ghana.
FAU - Senu, Ebenezer
AU  - Senu E
AD  - Department of Molecular Medicine, School of Medicine and Dentistry, Kwame Nkrumah 
      University of Science and Technology, Kumasi, Ghana.
FAU - Aidoo, Ebenezer
AU  - Aidoo E
AD  - Department of Medical Laboratory, Faculty of Applied Sciences, Accra Technical 
      University, Accra, Ghana.
FAU - Sarfo, Fred Stephen
AU  - Sarfo FS
AD  - Department of Medicine, School of Medicine and Dentistry, Kwame Nkrumah 
      University of Science and Technology, Komfo Anokye Teaching Hospital, Kumasi, 
      Ghana.
LA  - eng
PT  - Journal Article
DEP - 20221124
PL  - United States
TA  - Dialogues Health
JT  - Dialogues in health
JID - 9918506184906676
PMC - PMC10953871
OTO - NOTNLM
OT  - Cytokines
OT  - Hypertension
OT  - Inflammation
OT  - Interleukin-10
OT  - Stroke
OT  - monocyte chemoattractant protein-1 (MCP-1)
COIS- The authors declare that they have no competing interests.
EDAT- 2022/11/24 00:00
MHDA- 2022/11/24 00:01
PMCR- 2022/11/24
CRDT- 2024/03/22 04:02
PHST- 2022/06/11 00:00 [received]
PHST- 2022/11/17 00:00 [revised]
PHST- 2022/11/20 00:00 [accepted]
PHST- 2022/11/24 00:01 [medline]
PHST- 2022/11/24 00:00 [pubmed]
PHST- 2024/03/22 04:02 [entrez]
PHST- 2022/11/24 00:00 [pmc-release]
AID - S2772-6533(22)00086-7 [pii]
AID - 100086 [pii]
AID - 10.1016/j.dialog.2022.100086 [doi]
PST - epublish
SO  - Dialogues Health. 2022 Nov 24;1:100086. doi: 10.1016/j.dialog.2022.100086. 
      eCollection 2022 Dec.