PMID- 38517847
OWN - NLM
STAT- MEDLINE
DCOM- 20240325
LR  - 20240325
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 19
IP  - 3
DP  - 2024
TI  - Development and validation of multiplex one-step qPCR/RT-qPCR assays for 
      simultaneous detection of SARS-CoV-2 and pathogens associated with feline 
      respiratory disease complex.
PG  - e0297796
LID - 10.1371/journal.pone.0297796 [doi]
LID - e0297796
AB  - Feline respiratory disease complex (FRDC) is caused by a wide range of viral and 
      bacterial pathogens. Both Influenza A virus (IAV) and Severe Acute Respiratory 
      Syndrome Coronavirus-2 (SARS-CoV-2) also induce respiratory diseases in cats. Two 
      one-step multiplex qPCR/RT-qPCR assays were developed and validated: FRA_1 
      (Feline respiratory assay 1) for the detection of four viral targets and FRA_2 
      for the detection of three bacteria associated with FRDC. Both multiplex assays 
      demonstrated high specificity, efficiency (93.51%-107.8%), linearity (> 0.998), 
      analytical sensitivity (</= 15 genome copies/mul), repeatability (coefficient of 
      variation [CV] < 5%), and reproducibility (CV < 6%). Among the 63 clinical 
      specimens collected from FRDC-suspected cats, 92.1% were positive for at least 
      one pathogen and co-infection was detected in 57.1% of samples. Mycoplasma felis 
      (61.9%) was the most found pathogen, followed by feline herpesvirus-1 (30.2%), 
      Chlamydia felis (28.7%) and feline calicivirus (27.0%). SARS-CoV-2 was detected 
      in two specimens. In summary, this new panel of qPCR/RT-qPCR assays constitutes a 
      useful and reliable tool for the rapid detection of SARS-CoV-2 and viral and 
      bacterial pathogens associated with FRDC in cats.
CI  - Copyright: (c) 2024 Thieulent et al. This is an open access article distributed 
      under the terms of the Creative Commons Attribution License, which permits 
      unrestricted use, distribution, and reproduction in any medium, provided the 
      original author and source are credited.
FAU - Thieulent, Come J
AU  - Thieulent CJ
AUID- ORCID: 0000-0001-7402-5989
AD  - Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, Louisiana, United States of America.
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana 
      State University, Baton Rouge, Louisiana, United States of America.
FAU - Carossino, Mariano
AU  - Carossino M
AD  - Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, Louisiana, United States of America.
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana 
      State University, Baton Rouge, Louisiana, United States of America.
FAU - Peak, Laura
AU  - Peak L
AD  - Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, Louisiana, United States of America.
FAU - Wolfson, Wendy
AU  - Wolfson W
AD  - Department of Veterinary Clinical Sciences, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, Louisiana, United States of America.
FAU - Balasuriya, Udeni B R
AU  - Balasuriya UBR
AUID- ORCID: 0000-0003-0609-678X
AD  - Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, 
      Louisiana State University, Baton Rouge, Louisiana, United States of America.
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana 
      State University, Baton Rouge, Louisiana, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20240322
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Cats
MH  - Animals
MH  - SARS-CoV-2/genetics
MH  - Reproducibility of Results
MH  - *COVID-19/diagnosis
MH  - Bacteria/genetics
MH  - *Respiratory Tract Diseases
MH  - Sensitivity and Specificity
PMC - PMC10959388
COIS- The authors have declared that no competing interests exist.
EDAT- 2024/03/22 18:44
MHDA- 2024/03/25 06:43
PMCR- 2024/03/22
CRDT- 2024/03/22 13:33
PHST- 2023/08/01 00:00 [received]
PHST- 2024/01/12 00:00 [accepted]
PHST- 2024/03/25 06:43 [medline]
PHST- 2024/03/22 18:44 [pubmed]
PHST- 2024/03/22 13:33 [entrez]
PHST- 2024/03/22 00:00 [pmc-release]
AID - PONE-D-23-24264 [pii]
AID - 10.1371/journal.pone.0297796 [doi]
PST - epublish
SO  - PLoS One. 2024 Mar 22;19(3):e0297796. doi: 10.1371/journal.pone.0297796. 
      eCollection 2024.