PMID- 38521112
OWN - NLM
STAT- MEDLINE
DCOM- 20240426
LR  - 20240426
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 914
DP  - 2024 Jul 1
TI  - Identify CTBP1-DT as an immunological biomarker that promotes lipid synthesis and 
      apoptosis resistance in KIRC.
PG  - 148403
LID - S0378-1119(24)00284-1 [pii]
LID - 10.1016/j.gene.2024.148403 [doi]
AB  - Recently, mounting evidence has highlighted the essential function of the 
      C-terminal binding protein-1 divergent transcript (CTBP1-DT) in malignancies. 
      However, its role in kidney renal clear cell carcinoma (KIRC) remains largely 
      unknown. Our study aimed to identify the potential function of CTBP1-DT in KIRC. 
      RT-qPCR, Kaplan-Meier survival analysis, Cox regression analysis, and nomogram 
      analysis were utilized to determine the expression and effects of CTBP1-DT on 
      survival. The subcellular localization of CTBP1-DT was determined using RNA 
      fluorescence in situ hybridization (FISH). To investigate the functions of 
      CTBP1-DT in regulating KIRC cell proliferation, migration, invasion, lipid 
      synthesis, and apoptosis, we conducted CCK8, EdU, Transwell, and Oil Red O 
      staining and cell apoptosis staining assays. The relationships between CTBP1-DT 
      and the tumor microenvironment were investigated with multiple bioinformatics 
      analysis algorithms and databases, including CYBERSORT, TIMER2, Spearman 
      correlation test, tumor mutation burden (TMB), microsatellite instability (MSI), 
      and immunophenoscore (IPS). According to our results, CTBP1-DT is a lncRNA 
      located in the nucleus that is significantly upregulated in KIRC and is 
      correlated with better clinical outcomes. Downregulating CTBP1-DT inhibited cell 
      viability, migration, invasion, and lipid synthesis but triggered cell apoptosis. 
      Additionally, we explored the potential effect of CTBP1-DT in regulating immune 
      cell infiltration in KIRC and other malignancies. Furthermore, CTBP1-DT could be 
      used to predict the effectiveness of targeted drugs and immune checkpoint 
      inhibitors. In conclusion, we identified CTBP1-DT as a potential immunological 
      biomarker and discovered the potential role of CTBP1-DT in regulating lipid 
      synthesis and apoptosis resistance.
CI  - Copyright (c) 2024 Elsevier B.V. All rights reserved.
FAU - Li, Haolin
AU  - Li H
AD  - Department of Urology, The First Affiliated Hospital of Anhui Medical University, 
      Hefei, China.
FAU - Fei, Mintian
AU  - Fei M
AD  - Department of Urology, The First Affiliated Hospital of Anhui Medical University, 
      Hefei, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Department of Urology, The First Affiliated Hospital of Anhui Medical University, 
      Hefei, China.
FAU - Xu, Qili
AU  - Xu Q
AD  - Department of Urology, The First Affiliated Hospital of Anhui Medical University, 
      Hefei, China.
FAU - Feng, Rui
AU  - Feng R
AD  - Department of Urology, The First Affiliated Hospital of Anhui Medical University, 
      Hefei, China.
FAU - Cao, Jing
AU  - Cao J
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic 
      address: 2575241418@qq.com.
FAU - Qu, Yan
AU  - Qu Y
AD  - Department of Pathogenic Biology, School of Basic Medicine, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, 430030, China. 
      Electronic address: 382917561@qq.com.
FAU - Xiao, Haibing
AU  - Xiao H
AD  - Department of Urology, The First Affiliated Hospital of Anhui Medical University, 
      Hefei, China. Electronic address: xiaohaibing@ahmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240321
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - 0 (Biomarkers, Tumor)
RN  - EC 1.1.1.- (C-terminal binding protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Lipids)
SB  - IM
MH  - Humans
MH  - *Apoptosis
MH  - *Alcohol Oxidoreductases/genetics/metabolism
MH  - *Biomarkers, Tumor/genetics/metabolism
MH  - Cell Line, Tumor
MH  - *DNA-Binding Proteins/genetics/metabolism
MH  - *Cell Proliferation
MH  - *Carcinoma, Renal Cell/genetics/pathology/metabolism/immunology
MH  - Kidney Neoplasms/genetics/pathology/immunology/metabolism
MH  - Cell Movement
MH  - Gene Expression Regulation, Neoplastic
MH  - RNA, Long Noncoding/genetics
MH  - Lipids
MH  - Prognosis
MH  - Male
MH  - Female
OTO - NOTNLM
OT  - Apoptosis
OT  - CTBP1-DT
OT  - KIRC
OT  - Lipid
OT  - Tumor Microenvironment
OT  - lncRNA
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/03/24 00:42
MHDA- 2024/04/27 09:49
CRDT- 2024/03/23 20:36
PHST- 2023/11/23 00:00 [received]
PHST- 2024/03/03 00:00 [revised]
PHST- 2024/03/20 00:00 [accepted]
PHST- 2024/04/27 09:49 [medline]
PHST- 2024/03/24 00:42 [pubmed]
PHST- 2024/03/23 20:36 [entrez]
AID - S0378-1119(24)00284-1 [pii]
AID - 10.1016/j.gene.2024.148403 [doi]
PST - ppublish
SO  - Gene. 2024 Jul 1;914:148403. doi: 10.1016/j.gene.2024.148403. Epub 2024 Mar 21.