PMID- 38522207
OWN - NLM
STAT- MEDLINE
DCOM- 20240408
LR  - 20240408
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 469
DP  - 2024 May 5
TI  - Biomineralization of heavy metals based on urea transport and hydrolysis within a 
      new bacterial isolate, B. intermedia TSBOI.
PG  - 134049
LID - S0304-3894(24)00628-9 [pii]
LID - 10.1016/j.jhazmat.2024.134049 [doi]
AB  - A newly isolated ureolytic bacteria, Brucella intermedia TSBOI, exhibited 
      microbially induced calcite precipitation (MICP) which is a promising technique 
      for the remediation of heavy metals in polluted environments. Brucella intermedia 
      TSBOI achieved 90-100% removal of 1 mmol/L Cu(2+)/Pb(2+)/Zn(2+) within 72 h. A 
      distinctive feature lies in B. intermedia TSBOI's capacity for the transport and 
      hydrolysis of urea, considered to be critical for its strong urease activity. 
      This study explored the mechanisms of this capacity at the genetic, molecular and 
      protein levels through complete genome sequencing, molecular docking and 
      enzymatic reaction kinetics. The results revealed that, for urea hydrolysis, B. 
      intermedia TSBOI exhibited a comprehensive urease gene cluster, with the key gene 
      ureC demonstrating an absolute expression level approximating to 4 x 10(4) 
      copies/RNA ng under optimal conditions. Results also confirmed the strong 
      spontaneous, energy-independent binding ability of it's urease to urea, with the 
      lowest Gibbs free energy binding site linking to the three amino acids, alanine, 
      asparagine and serine. The urea transport gene yut presented and expressed, with 
      the absolute expression enhanced in response to increasing urea concentrations. 
      The significant positive correlation between ureC/yut expression levels and 
      urease activity provided a theoretical basis for B. intermedia TSBOI's heavy 
      metal bioremediation potential. ENVIRONMENTAL IMPLICATION: Heavy metals (Cu, Pb 
      and Zn) were studied in this study. Heavy metals are hazardous due to their 
      toxicity, persistence, and ability to bioaccumulate in living organisms. They can 
      cause severe health issues, harm ecosystems, and contaminate air, water, and 
      soil. A novel ureolytic bacteria, Brucella intermedia TSBOI, exhibited 
      microbially induced carbonate precipitation capability was isolated which removed 
      90-100% of 1 mmol/L Cu(2+)/Pb(2+)/Zn(2+) within 72 h. Its advantages in urea 
      hydrolysis and transport facilitate the remediation of actual heavy metal 
      contaminated environments.
CI  - Copyright (c) 2024 Elsevier B.V. All rights reserved.
FAU - Hu, Xuesong
AU  - Hu X
AD  - School of Chemical & Environmental Engineering, China University of Mining and 
      Technology (Beijing), 100083 Beijing, China.
FAU - Yu, Caihong
AU  - Yu C
AD  - School of Chemical & Environmental Engineering, China University of Mining and 
      Technology (Beijing), 100083 Beijing, China. Electronic address: 
      caihongyu@cumtb.edu.cn.
FAU - Li, Xianhong
AU  - Li X
AD  - School of Chemical & Environmental Engineering, China University of Mining and 
      Technology (Beijing), 100083 Beijing, China.
FAU - Zou, Jiacheng
AU  - Zou J
AD  - School of Chemical & Environmental Engineering, China University of Mining and 
      Technology (Beijing), 100083 Beijing, China.
FAU - Du, Yanbin
AU  - Du Y
AD  - School of Chemical & Environmental Engineering, China University of Mining and 
      Technology (Beijing), 100083 Beijing, China.
FAU - Paterson, David M
AU  - Paterson DM
AD  - Scottish Oceans Institute, School of Biology, University of St Andrews, East 
      Sands, St. Andrews, Fife KY16 8LB, UK.
LA  - eng
PT  - Journal Article
DEP - 20240316
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - EC 3.5.1.5 (Urease)
RN  - 2P299V784P (Lead)
RN  - 0 (Metals, Heavy)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 0 (Soil)
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - *Ecosystem
MH  - Urease/metabolism
MH  - Biomineralization
MH  - Hydrolysis
MH  - Lead/metabolism
MH  - Molecular Docking Simulation
MH  - *Metals, Heavy/metabolism
MH  - Calcium Carbonate/chemistry
MH  - Bacteria/metabolism
MH  - Soil/chemistry
MH  - Urea/metabolism
OTO - NOTNLM
OT  - Comparative genomics
OT  - Molecular docking
OT  - RT-qPCR
OT  - Urea transporter
OT  - ureC expression
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/03/25 00:42
MHDA- 2024/04/08 06:43
CRDT- 2024/03/24 19:06
PHST- 2023/12/21 00:00 [received]
PHST- 2024/03/12 00:00 [revised]
PHST- 2024/03/13 00:00 [accepted]
PHST- 2024/04/08 06:43 [medline]
PHST- 2024/03/25 00:42 [pubmed]
PHST- 2024/03/24 19:06 [entrez]
AID - S0304-3894(24)00628-9 [pii]
AID - 10.1016/j.jhazmat.2024.134049 [doi]
PST - ppublish
SO  - J Hazard Mater. 2024 May 5;469:134049. doi: 10.1016/j.jhazmat.2024.134049. Epub 
      2024 Mar 16.