PMID- 38522660
OWN - NLM
STAT- MEDLINE
DCOM- 20240427
LR  - 20240427
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 545
DP  - 2024 May 3
TI  - Electroacupuncture Alleviates Post-stroke Cognitive Impairment Through Inhibiting 
      miR-135a-5p/mTOR/NLRP3 Axis-mediated Autophagy.
PG  - 185-195
LID - S0306-4522(24)00119-2 [pii]
LID - 10.1016/j.neuroscience.2024.03.008 [doi]
AB  - Post-stroke cognitive impairment is a significant challenge with limited 
      treatment options. Electroacupuncture (EA) has shown promise in improving 
      cognitive function after stroke. Our study explores the underlying mechanism of 
      EA in alleviating cognitive impairment through the inhibition of autophagy. We 
      utilized a rat model of stroke induced by middle cerebral artery occlusion (MCAO) 
      to evaluate the efficacy of EA. Treatment with EA was observed to markedly 
      improve cognitive function and reduce inflammation in MCAO rats, as evidenced by 
      decreased neurological deficit scores, shorter latencies in the water maze test, 
      and diminished infarct volumes. EA also attenuated tissue damage in the 
      hippocampus and lowered the levels of pro-inflammatory cytokines and oxidative 
      stress markers. Although autophagy was upregulated in MCAO rats, EA treatment 
      suppressed this process, indicated by a reduction in autophagosome formation and 
      alteration of autophagy-related protein expression. The protective effects of EA 
      were reversed by the autophagy activator rapamycin. EA treatment elevated the 
      levels of microRNA (miR)-135a-5p expression, and suppression of this elevation 
      attenuated the remedial efficacy of EA in addressing cognitive impairment and 
      inflammation. MiR-135a-5p targeted mammalian target of rapamycin (mTOR)/NOD-like 
      receptor protein 3 (NLRP3) signaling to repress autophagy. EA treatment inhibits 
      autophagy and alleviates cognitive impairment in post-stroke rats. It exerts its 
      beneficial effects by upregulating miR-135a-5p and targeting the mTOR/NLRP3 axis.
CI  - Copyright (c) 2024 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Luo, Jianchang
AU  - Luo J
AD  - Department of Rehabilitation Medicine, Taizhou Municipal Hospital, Taizhou 
      318000, China. Electronic address: tz20190331@163.com.
FAU - Lang, Jiawang
AU  - Lang J
AD  - Department of Rehabilitation Medicine, Taizhou Municipal Hospital, Taizhou 
      318000, China. Electronic address: ljw88289722@163.com.
FAU - Xu, Wenbin
AU  - Xu W
AD  - Department of Rehabilitation Medicine, Taizhou Municipal Hospital, Taizhou 
      318000, China. Electronic address: uiyt678@126.com.
FAU - Wang, Luodan
AU  - Wang L
AD  - Department of Rehabilitation Medicine, Taizhou Municipal Hospital, Taizhou 
      318000, China. Electronic address: fengslw@163.com.
FAU - Zhao, Zhipeng
AU  - Zhao Z
AD  - Department of Rehabilitation Medicine, School of Medicine, Taizhou University, 
      Taizhou 318000, China. Electronic address: Zhaozhipeng0129@163.com.
FAU - Jia, Jie
AU  - Jia J
AD  - Department of Rehabilitation Medicine, Huashan Hospital Affiliated to Fudan 
      University, Shanghai 200040, China. Electronic address: shannonjj@126.com.
FAU - Lang, Boxu
AU  - Lang B
AD  - Department of Rehabilitation Medicine, Taizhou Municipal Hospital, Taizhou 
      318000, China. Electronic address: tsylbx@tzc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20240322
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (MicroRNAs)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (Nlrp3 protein, rat)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - 0 (MIRN135 microRNA, rat)
SB  - IM
MH  - Animals
MH  - *Electroacupuncture/methods
MH  - *MicroRNAs/metabolism
MH  - *Autophagy/physiology
MH  - *NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - *TOR Serine-Threonine Kinases/metabolism
MH  - Male
MH  - *Cognitive Dysfunction/etiology/therapy/metabolism
MH  - *Rats, Sprague-Dawley
MH  - *Stroke/metabolism/complications/therapy
MH  - Rats
MH  - Infarction, Middle Cerebral Artery/therapy/complications/metabolism
MH  - Signal Transduction/physiology
MH  - Hippocampus/metabolism
MH  - Disease Models, Animal
OTO - NOTNLM
OT  - NLRP3
OT  - autophagy
OT  - electroacupuncture
OT  - mTOR
OT  - miR-135a-5p
OT  - post-stroke cognitive impairment
EDAT- 2024/03/25 00:42
MHDA- 2024/04/28 07:24
CRDT- 2024/03/24 20:28
PHST- 2023/09/15 00:00 [received]
PHST- 2024/03/08 00:00 [revised]
PHST- 2024/03/11 00:00 [accepted]
PHST- 2024/04/28 07:24 [medline]
PHST- 2024/03/25 00:42 [pubmed]
PHST- 2024/03/24 20:28 [entrez]
AID - S0306-4522(24)00119-2 [pii]
AID - 10.1016/j.neuroscience.2024.03.008 [doi]
PST - ppublish
SO  - Neuroscience. 2024 May 3;545:185-195. doi: 10.1016/j.neuroscience.2024.03.008. 
      Epub 2024 Mar 22.