PMID- 38525131
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240326
IS  - 1976-8257 (Print)
IS  - 2234-2753 (Electronic)
IS  - 1976-8257 (Linking)
VI  - 40
IP  - 2
DP  - 2024 Apr
TI  - n-Acetylcysteine protects against diazinon-induced histopathological damage and 
      apoptosis in renal tissue of rats.
PG  - 285-295
LID - 10.1007/s43188-024-00226-3 [doi]
AB  - Diazinon (DZN) is a member of organophosphorus insecticides that has cytotoxic 
      effects on different organs. n-Acetyl cysteine (NAC) is a widely used antioxidant 
      in clinical, in vivo and in vitro studies. We evaluated the protective role of 
      NAC against DZN-induced toxicity in kidney tissue of Wistar rats. 30 male Wistar 
      rats were divided into 5 groups of control, single dose of DZN, continuous dose 
      of DZN, single doses of DZN + NAC and continuous doses of DZN + NAC. Kidney 
      function test (blood urea nitrogen, creatinine and uric acid) was provided. 
      Levels of malondialdehyde (MDA), total antioxidant capacity (TAC) and total 
      sulfhydryl (T-SH) were determined in renal tissues. Renal cells apoptosis was 
      detected using TUNEL assay. The mRNA expressions of apoptosis, oxidative stress 
      and inflammatory mediators, including B-cell lymphoma-2 (Bcl2), Bcl-2-associated 
      X protein (Bax), superoxide dismutase (SOD), catalase (CAT), Interleukin 10 
      (IL-10), Tumor necrosis factor-alpha (TNF-alpha), Caspase-3 and Caspase-8 were analyzed 
      in kidney tissues using Real Time PCR method. Chronic exposure to DZN was 
      associated with severe morphological changes in the kidney, as well as impairment 
      of its function and decreased kidney weights. Continues treatment with DZN 
      significantly decreased the percentage of renal apoptotic cells as compared to 
      rats treated with continuous dose of DZN alone (17.69 +/- 3.67% vs. 39.46% +/- 2.44%; 
      p < 0.001). Continuous exposure to DZN significantly decreased TAC and T-SH 
      contents, as well as SOD and CAT expression, but increased MDA contents in the 
      kidney tissues (p < 0.001). A significant increase was observed in mRNA 
      expression of Bax, Caspase-3, Caspase-8, as well as TNF-alpha following exposure to 
      DZN, but the expression of IL-10 and Bcl2 was significantly decreased. NAC can 
      protect kidney tissue against DZN-induced toxicity by elevating antioxidants 
      capacity, mitigating oxidative stress, inflammation and apoptosis.
CI  - (c) The Author(s) under exclusive licence to Korean Society of Toxicology 2024. 
      Springer Nature or its licensor (e.g. a society or other partner) holds exclusive 
      rights to this article under a publishing agreement with the author(s) or other 
      rightsholder(s); author self-archiving of the accepted manuscript version of this 
      article is solely governed by the terms of such publishing agreement and 
      applicable law.
FAU - Dong, Gaiqin
AU  - Dong G
AD  - Department of Nephrology and Immunology, Children's Hospital of Soochow 
      University, Suzhou, 225001 Jiangsu China. GRID: grid.452253.7. ISNI: 0000 0004 
      1804 524X
AD  - Department of Pediatrics, Affiliated Hospital of Yangzhou University, Yangzhou, 
      225000 Jiangsu China. ROR: https://ror.org/03tqb8s11. GRID: grid.268415.c
FAU - Li, Qingfeng
AU  - Li Q
AD  - Department of Pediatrics, Affiliated Hospital of Yangzhou University, Yangzhou, 
      225000 Jiangsu China. ROR: https://ror.org/03tqb8s11. GRID: grid.268415.c
FAU - Yu, Chun
AU  - Yu C
AD  - Department of Pediatrics, Affiliated Hospital of Yangzhou University, Yangzhou, 
      225000 Jiangsu China. ROR: https://ror.org/03tqb8s11. GRID: grid.268415.c
FAU - Wang, Qing
AU  - Wang Q
AD  - Department of Pediatrics, Affiliated Hospital of Yangzhou University, Yangzhou, 
      225000 Jiangsu China. ROR: https://ror.org/03tqb8s11. GRID: grid.268415.c
FAU - Zuo, Danhua
AU  - Zuo D
AD  - Department of Pediatrics, Affiliated Hospital of Yangzhou University, Yangzhou, 
      225000 Jiangsu China. ROR: https://ror.org/03tqb8s11. GRID: grid.268415.c
FAU - Li, Xiaozhong
AU  - Li X
AUID- ORCID: 0009-0006-5951-9669
AD  - Department of Nephrology and Immunology, Children's Hospital of Soochow 
      University, Suzhou, 225001 Jiangsu China. GRID: grid.452253.7. ISNI: 0000 0004 
      1804 524X
LA  - eng
PT  - Journal Article
DEP - 20240307
PL  - Singapore
TA  - Toxicol Res
JT  - Toxicological research
JID - 101483324
PMC - PMC10959863
OTO - NOTNLM
OT  - Apoptosis
OT  - Diazinon
OT  - Kidney tissue
OT  - Oxidative stress
OT  - n-Acetylcysteine
COIS- Conflict of interestThe authors declare that they have no conflicts of interest.
EDAT- 2024/03/25 06:42
MHDA- 2024/03/25 06:43
PMCR- 2025/04/01
CRDT- 2024/03/25 04:37
PHST- 2023/08/29 00:00 [received]
PHST- 2023/11/04 00:00 [revised]
PHST- 2024/01/22 00:00 [accepted]
PHST- 2025/04/01 00:00 [pmc-release]
PHST- 2024/03/25 06:43 [medline]
PHST- 2024/03/25 06:42 [pubmed]
PHST- 2024/03/25 04:37 [entrez]
AID - 226 [pii]
AID - 10.1007/s43188-024-00226-3 [doi]
PST - epublish
SO  - Toxicol Res. 2024 Mar 7;40(2):285-295. doi: 10.1007/s43188-024-00226-3. 
      eCollection 2024 Apr.