PMID- 38526189
OWN - NLM
STAT- MEDLINE
DCOM- 20240409
LR  - 20240425
IS  - 2046-6390 (Electronic)
IS  - 2046-6390 (Linking)
VI  - 13
IP  - 4
DP  - 2024 Apr 15
TI  - Cdc48 and its co-factor Ufd1 extract CENP-A from centromeric chromatin and can 
      induce chromosome elimination in the fission yeast Schizosaccharomyces pombe.
LID - 10.1242/bio.060287 [doi]
LID - bio060287
AB  - CENP-A determines the identity of the centromere. Because the position and size 
      of the centromere and its number per chromosome must be maintained, the 
      distribution of CENP-A is strictly regulated. In this study, we have aimed to 
      understand mechanisms to regulate the distribution of CENP-A (Cnp1SP) in fission 
      yeast. A mutant of the ufd1+ gene (ufd1-73) encoding a cofactor of Cdc48 ATPase 
      is sensitive to Cnp1 expressed at a high level and allows mislocalization of 
      Cnp1. The level of Cnp1 in centromeric chromatin is increased in the ufd1-73 
      mutant even when Cnp1 is expressed at a normal level. A preexisting mutant of the 
      cdc48+ gene (cdc48-353) phenocopies the ufd1-73 mutant. We have also shown that 
      Cdc48 and Ufd1 proteins interact physically with centromeric chromatin. Finally, 
      Cdc48 ATPase with Ufd1 artificially recruited to the centromere of a 
      mini-chromosome (Ch16) induce a loss of Cnp1 from Ch16, leading to an increased 
      rate of chromosome loss. It appears that Cdc48 ATPase, together with its cofactor 
      Ufd1 remove excess Cnp1 from chromatin, likely in a direct manner. This mechanism 
      may play a role in centromere disassembly, a process to eliminate Cnp1 to 
      inactivate the kinetochore function during development, differentiation, and 
      stress response.
CI  - (c) 2024. Published by The Company of Biologists Ltd.
FAU - Nakase, Yukiko
AU  - Nakase Y
AUID- ORCID: 0000-0002-2669-5539
AD  - Radiation Biology Center, Graduate School of Biostudies, Kyoto University, 
      Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan.
FAU - Murakami, Hiroaki
AU  - Murakami H
AUID- ORCID: 0000-0003-0283-7106
AD  - Radiation Biology Center, Graduate School of Biostudies, Kyoto University, 
      Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan.
FAU - Suma, Michiko
AU  - Suma M
AUID- ORCID: 0009-0009-7414-7557
AD  - Radiation Biology Center, Graduate School of Biostudies, Kyoto University, 
      Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan.
FAU - Nagano, Kaho
AU  - Nagano K
AD  - Radiation Biology Center, Graduate School of Biostudies, Kyoto University, 
      Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan.
FAU - Wakuda, Airi
AU  - Wakuda A
AD  - Radiation Biology Center, Graduate School of Biostudies, Kyoto University, 
      Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan.
FAU - Kitagawa, Teppei
AU  - Kitagawa T
AD  - Radiation Biology Center, Graduate School of Biostudies, Kyoto University, 
      Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan.
FAU - Matsumoto, Tomohiro
AU  - Matsumoto T
AUID- ORCID: 0000-0001-6199-5126
AD  - Radiation Biology Center, Graduate School of Biostudies, Kyoto University, 
      Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan.
LA  - eng
GR  - 20K06485/Japan Society for the Promotion of Science/
GR  - Kyoto University/
PT  - Journal Article
DEP - 20240408
PL  - England
TA  - Biol Open
JT  - Biology open
JID - 101578018
RN  - 0 (Chromatin)
RN  - 0 (Centromere Protein A)
RN  - 0 (Histones)
RN  - 0 (Schizosaccharomyces pombe Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - 0 (Plant Extracts)
RN  - 0 (Cnp1 protein, S pombe)
SB  - IM
MH  - Chromatin/genetics/metabolism
MH  - *Schizosaccharomyces/genetics/metabolism
MH  - Centromere Protein A/genetics/metabolism
MH  - Histones/metabolism
MH  - *Schizosaccharomyces pombe Proteins/genetics/metabolism
MH  - Chromosomal Proteins, Non-Histone/genetics/metabolism
MH  - Centromere/genetics/metabolism
MH  - Adenosine Triphosphatases/metabolism
MH  - Plant Extracts/metabolism
PMC - PMC11033524
OTO - NOTNLM
OT  - Cdc48
OT  - Centromere
OT  - Chromosome
OT  - Cnp1
OT  - Pombe
OT  - Ufd1
COIS- Competing interests The authors declare no competing or financial interests.
EDAT- 2024/03/25 12:43
MHDA- 2024/04/09 06:45
PMCR- 2024/04/08
CRDT- 2024/03/25 09:34
PHST- 2023/12/19 00:00 [received]
PHST- 2024/03/14 00:00 [accepted]
PHST- 2024/04/09 06:45 [medline]
PHST- 2024/03/25 12:43 [pubmed]
PHST- 2024/03/25 09:34 [entrez]
PHST- 2024/04/08 00:00 [pmc-release]
AID - 345663 [pii]
AID - BIO060287 [pii]
AID - 10.1242/bio.060287 [doi]
PST - ppublish
SO  - Biol Open. 2024 Apr 15;13(4):bio060287. doi: 10.1242/bio.060287. Epub 2024 Apr 8.