PMID- 38532720
OWN - NLM
STAT- MEDLINE
DCOM- 20240328
LR  - 20240508
IS  - 1525-6049 (Electronic)
IS  - 0886-022X (Print)
IS  - 0886-022X (Linking)
VI  - 45
IP  - 2
DP  - 2023
TI  - Increased expression of the P2Y(12) receptor is involved in the failure of 
      autogenous arteriovenous fistula caused by stenosis.
PG  - 2278314
LID - 10.1080/0886022X.2023.2278314 [doi]
LID - 2278314
AB  - OBJECTIVE: This study investigated the role of the P2Y(12) receptor in autogenous 
      arteriovenous fistula (AVF) failure resulting from stenosis. METHODS: Stenotic 
      venous tissues and blood samples were obtained from patients with end-stage renal 
      disease (ESRD) together with AVF stenosis, while venous tissues and blood samples 
      were collected from patients with ESRD undergoing initial AVF surgery as 
      controls. Immunohistochemistry and/or immunofluorescence techniques were utilized 
      to assess the expression of P2Y(12), transforming growth factor-beta1 (TGF-beta1), 
      monocyte chemotactic protein 1 (MCP-1), and CD68 in the venous tissues. The 
      expression levels of P2Y(12), TGFbeta1, and MCP-1 were quantified using quantitative 
      reverse transcription-polymerase chain reaction and western blot analyses. Double 
      and triple immunofluorescence staining was performed to precisely localize the 
      cellular localization of P2Y(12) expression. RESULTS: Expression levels of 
      P2Y(12), TGFbeta1, MCP-1, and CD68 were significantly higher in stenotic AVF venous 
      tissues than in the control group tissues. Double and triple immunofluorescence 
      staining of stenotic AVF venous tissues indicated that P2Y(12) was predominantly 
      expressed in alpha-SMA-positive vascular smooth muscle cells (VSMCs) and, to a lesser 
      extent, in CD68-positive macrophages, with limited expression in CD31-positive 
      endothelial cells. Moreover, a subset of macrophage-like VSMCs expressing P2Y(12) 
      were observed in both stenotic AVF venous tissues and control venous tissues. 
      Additionally, a higher number of P2Y(12)(+)/TGF-beta1(+) double-positive cells were 
      identified in stenotic AVF venous tissues than in the control group tissues. 
      CONCLUSION: Increased expression of P2Y(12) in stenotic AVF venous tissues of 
      patients with ESRD suggests its potential involvement in the pathogenesis of 
      venous stenosis within AVFs.
FAU - Liu, Lei
AU  - Liu L
AD  - Department of Nephrology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
AD  - Department of Nephrology, Chongqing University Three Gorges Hospital, Chongqing, 
      China.
AD  - Department of Nephrology, Chongqing Three Gorges Central Hospital, Chongqing, 
      China.
FAU - Gao, Jianya
AU  - Gao J
AD  - Department of Nephrology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
AD  - Department of Nephrology, Chongqing University Three Gorges Hospital, Chongqing, 
      China.
AD  - Department of Nephrology, Chongqing Three Gorges Central Hospital, Chongqing, 
      China.
FAU - Tang, Yuewu
AU  - Tang Y
AD  - Department of Nephrology, Chongqing University Three Gorges Hospital, Chongqing, 
      China.
AD  - Department of Nephrology, Chongqing Three Gorges Central Hospital, Chongqing, 
      China.
FAU - Guo, Guangfeng
AU  - Guo G
AD  - Department of Nephrology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Gan, Hua
AU  - Gan H
AD  - Department of Nephrology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20231123
PL  - England
TA  - Ren Fail
JT  - Renal failure
JID - 8701128
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Humans
MH  - Renal Dialysis
MH  - Constriction, Pathologic
MH  - Endothelial Cells
MH  - Transforming Growth Factor beta1
MH  - *Arteriovenous Shunt, Surgical
MH  - *Kidney Failure, Chronic
MH  - *Arteriovenous Fistula
PMC - PMC11073481
OTO - NOTNLM
OT  - P2Y12
OT  - arteriovenous fistula (AVF)
OT  - hemodialysis
OT  - neointima
OT  - stenosis
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2023/01/01 00:00
MHDA- 2024/03/28 06:44
PMCR- 2023/11/23
CRDT- 2024/03/27 03:43
PHST- 2024/03/28 06:44 [medline]
PHST- 2023/01/01 00:00 [pubmed]
PHST- 2024/03/27 03:43 [entrez]
PHST- 2023/11/23 00:00 [pmc-release]
AID - 2278314 [pii]
AID - 10.1080/0886022X.2023.2278314 [doi]
PST - ppublish
SO  - Ren Fail. 2023;45(2):2278314. doi: 10.1080/0886022X.2023.2278314. Epub 2023 Nov 
      23.