PMID- 38534459
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 13
IP  - 3
DP  - 2024 Mar 15
TI  - The Effects of Salinity and Genotype on the Rhizospheric Mycobiomes in Date Palm 
      Seedlings.
LID - 10.3390/biology13030190 [doi]
LID - 190
AB  - Salinity severely affects the health and productivity of plants, with 
      root-associated microbes, including fungi, potentially playing a crucial role in 
      mitigating this effect and promoting plant health. This study employed 
      metagenomics to investigate differences in the structures of the epiphyte 
      mycobiomes in the rhizospheres of seedlings of two distinct date palm cultivars 
      with contrasting salinity tolerances, the susceptible cultivar, 'Zabad', and the 
      tolerant cultivar, 'Umsila'. Next-generation sequencing (NGS) of the internal 
      transcribed spacer (ITS) rRNA was utilized as a DNA barcoding tool. The 
      sequencing of 12 mycobiome libraries yielded 905,198 raw sequences of 268,829 
      high-quality reads that coded for 135 unique and annotatable operational 
      taxonomic units (OTUs). An OTU analysis revealed differences in the rhizofungal 
      community structures between the treatments regardless of genotype, and 
      non-metric dimensional scaling (N-MDS) analyses demonstrated distinct separations 
      between the cultivars under saline stress. However, these differences were not 
      detected under the control environmental conditions, i.e., no salinity. The 
      rhizospheric fungal community included four phyla (Ascomycota, Basidiomycota, 
      Chytridiomycota, and Mucoromycota), with differences in the abundances of 
      Aspergillus, Clonostachys, and Fusarium genera in response to salinity, 
      regardless of the genotype. Differential pairwise comparisons showed that 
      Fusarium falciforme-solani and Aspergillus sydowii-versicolor increased in 
      abundance under saline conditions, providing potential future in vitro isolation 
      guidelines for plant growth-promoting fungi. This study highlights the intricate 
      dynamics of the rhizosphere microbial communities in date palms and their 
      responses to salt stress. Additionally, we found no support for the hypothesis 
      that indigenous epiphytic fungal communities are significantly involved in 
      salinity tolerance in date palms.
FAU - Yaish, Mahmoud W
AU  - Yaish MW
AUID- ORCID: 0000-0002-5171-3014
AD  - Department of Biology, College of Sciences, Sultan Qaboos University, P.O. Box 
      36, Muscat 123, Oman.
FAU - Al-Busaidi, Aya
AU  - Al-Busaidi A
AD  - Department of Biology, College of Sciences, Sultan Qaboos University, P.O. Box 
      36, Muscat 123, Oman.
FAU - Glick, Bernard R
AU  - Glick BR
AUID- ORCID: 0000-0003-0193-158X
AD  - Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
FAU - Ahmed, Talaat
AU  - Ahmed T
AUID- ORCID: 0000-0001-8022-1855
AD  - Environmental Science Center, Qatar University, Doha P.O. Box 2713, Qatar.
FAU - Alatalo, Juha M
AU  - Alatalo JM
AUID- ORCID: 0000-0001-5084-850X
AD  - Environmental Science Center, Qatar University, Doha P.O. Box 2713, Qatar.
LA  - eng
GR  - IG/SCI/BIOL/24/03/Sultan Qaboos University/
PT  - Journal Article
DEP - 20240315
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC10968608
OTO - NOTNLM
OT  - NGS
OT  - PGPB
OT  - cultivars
OT  - date palm
OT  - mycobiomes
OT  - rhizofungus
OT  - salinity
COIS- The authors declare no conflicts of interest.
EDAT- 2024/03/27 12:50
MHDA- 2024/03/27 12:51
PMCR- 2024/03/15
CRDT- 2024/03/27 09:25
PHST- 2024/02/21 00:00 [received]
PHST- 2024/03/12 00:00 [revised]
PHST- 2024/03/14 00:00 [accepted]
PHST- 2024/03/27 12:51 [medline]
PHST- 2024/03/27 12:50 [pubmed]
PHST- 2024/03/27 09:25 [entrez]
PHST- 2024/03/15 00:00 [pmc-release]
AID - biology13030190 [pii]
AID - biology-13-00190 [pii]
AID - 10.3390/biology13030190 [doi]
PST - epublish
SO  - Biology (Basel). 2024 Mar 15;13(3):190. doi: 10.3390/biology13030190.