PMID- 38536233
OWN - NLM
STAT- MEDLINE
DCOM- 20240328
LR  - 20240407
IS  - 2057-5858 (Electronic)
IS  - 2057-5858 (Linking)
VI  - 10
IP  - 3
DP  - 2024 Mar
TI  - Integrative methylome and transcriptome analysis reveals epigenetic regulation of 
      Fusobacterium nucleatum in laryngeal cancer.
LID - 10.1099/mgen.0.001221 [doi]
LID - 001221
AB  - The aetiological mechanisms of Fusobacterium nucleatum in laryngeal cancer remain 
      unclear. This study aimed to reveal the epigenetic signature induced by F. 
      nucleatum in laryngeal squamous cell carcinoma (LSCC). Combined analysis of 
      methylome and transcriptome data was performed to address the functional role of 
      F. nucleatum in laryngeal cancer. Twenty-nine differentially expressed 
      methylation-driven genes were identified by mapping the methylation levels of 
      significant differential methylation sites to the expression levels of related 
      genes. The combined analysis revealed that F. nucleatum promoted Janus kinase 3 
      (JAK3) gene expression in LSCC. Further validation found decreased methylation 
      and elevated expression of JAK3 in the F. nucleatum-treated LSCC cell group; F. 
      nucleatum abundance and JAK3 gene expression had a positive correlation in tumour 
      tissues. This analysis provides a novel understanding of the impact of F. 
      nucleatum in the methylome and transcriptome of laryngeal cancer. Identification 
      of these epigenetic regulatory mechanisms opens up new avenues for mechanistic 
      studies to explore novel therapeutic strategies.
FAU - Yuan, Xiaohui
AU  - Yuan X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital, 
      Fudan University, Shanghai, PR China.
AD  - Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, PR China.
FAU - Lau, Hui-Ching
AU  - Lau HC
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital, 
      Fudan University, Shanghai, PR China.
AD  - Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, PR China.
FAU - Huang, Huiying
AU  - Huang H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital, 
      Fudan University, Shanghai, PR China.
AD  - Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, PR China.
FAU - Hsueh, Chi-Yao
AU  - Hsueh CY
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital, 
      Fudan University, Shanghai, PR China.
AD  - Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, PR China.
FAU - Gong, Hongli
AU  - Gong H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital, 
      Fudan University, Shanghai, PR China.
AD  - Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, PR China.
FAU - Zhou, Liang
AU  - Zhou L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye and ENT Hospital, 
      Fudan University, Shanghai, PR China.
AD  - Shanghai Key Clinical Disciplines of Otorhinolaryngology, Shanghai, PR China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Microb Genom
JT  - Microbial genomics
JID - 101671820
SB  - IM
MH  - Humans
MH  - *Epigenome
MH  - Fusobacterium nucleatum
MH  - *Laryngeal Neoplasms
MH  - Epigenesis, Genetic
MH  - Gene Expression Profiling
PMC - PMC10995630
OTO - NOTNLM
OT  - Fusobacterium nucleatum
OT  - functional enrichment analysis
OT  - laryngeal cancer
OT  - methylome
OT  - transcriptome
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2024/03/27 12:50
MHDA- 2024/03/28 06:45
PMCR- 2024/03/27
CRDT- 2024/03/27 11:54
PHST- 2024/03/28 06:45 [medline]
PHST- 2024/03/27 12:50 [pubmed]
PHST- 2024/03/27 11:54 [entrez]
PHST- 2024/03/27 00:00 [pmc-release]
AID - 001221 [pii]
AID - 10.1099/mgen.0.001221 [doi]
PST - ppublish
SO  - Microb Genom. 2024 Mar;10(3):001221. doi: 10.1099/mgen.0.001221.