PMID- 38537374
OWN - NLM
STAT- MEDLINE
DCOM- 20240405
LR  - 20240405
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 19
IP  - 3
DP  - 2024 Apr 4
TI  - Enhancing osteoblast proliferation and bone regeneration by poly (amino 
      acid)/selenium-doped hydroxyapatite.
LID - 10.1088/1748-605X/ad38ac [doi]
AB  - Among various biomaterials employed for bone repair, composites with good 
      biocompatibility and osteogenic ability had received increasing attention from 
      biomedical applications. In this study, we doped selenium (Se) into 
      hydroxyapatite (Se-HA) by the precipitation method, and prepared different 
      amounts of Se-HA-loaded poly (amino acid)/Se-HA (PAA/Se-HA) composites (0, 10 
      wt%, 20 wt%, 30 wt%) byin-situmelting polycondensation. The physical and chemical 
      properties of PAA/Se-HA composites were characterized by x-ray diffraction (XRD), 
      Fourier transform infrared spectroscopy (FT-IR), differential scanning 
      calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy 
      (SEM) and their mechanical properties. XRD and FT-IR results showed that 
      PAA/Se-HA composites contained characteristic peaks of PAA and Se-HA with amide 
      linkage and HA structures. DSC and TGA results specified the PAA/Se-HA30 
      composite crystallization, melting, and maximum weight loss temperatures at 
      203.33  degrees C, 162.54  degrees C, and 468.92  degrees C, respectively, which implied good thermal 
      stability. SEM results showed that Se-HA was uniformly dispersed in PAA. The 
      mechanical properties of PAA/Se-HA30 composites included bending, compressive, 
      and yield strengths at 83.07 +/- 0.57, 106.56 +/- 0.46, and 99.17 +/- 1.11 MPa, 
      respectively. The cellular responses of PAA/Se-HA compositesin vitrowere studied 
      using bone marrow mesenchymal stem cells (BMSCs) by cell counting kit-8 assay, 
      and results showed that PAA/Se-HA30 composites significantly promoted the 
      proliferation of BMSCs at the concentration of 2 mg ml(-1). The alkaline 
      phosphatase activity (ALP) and alizarin red staining results showed that the 
      introduction of Se-HA into PAA enhanced ALP activity and formation of calcium 
      nodule. Western blotting and Real-time polymerase chain reaction results showed 
      that the introduction of Se-HA into PAA could promoted the expression of 
      osteogenic-related proteins and mRNA (integrin-binding sialoprotein, osteopontin, 
      runt-related transcription factor 2 and Osterix) in BMSCs. A muscle defect at the 
      back and a bone defect at the femoral condyle of New Zealand white rabbits were 
      introduced for evaluating the enhancement of bone regeneration of PAA and 
      PAA/Se-HA30 composites. The implantation of muscle tissue revealed good 
      biocompatibility of PAA and PAA/Se-HA30 composites. The implantation of bone 
      defect showed that PAA/Se-HA30 composites enhanced bone formation at the defect 
      site (8 weeks), exhibiting good bone conductivity. Therefore, the PAA-based 
      composite was a promising candidate material for bone tissue regeneration.
CI  - (c) 2024 IOP Publishing Ltd.
FAU - Wei, Xiaobo
AU  - Wei X
AD  - Medical College, Yan'an University, Yan'an 716000, People's Republic of China.
FAU - Zhang, Ziyue
AU  - Zhang Z
AD  - Medical College, Yan'an University, Yan'an 716000, People's Republic of China.
FAU - Wang, Lei
AU  - Wang L
AD  - Medical College, Yan'an University, Yan'an 716000, People's Republic of China.
FAU - Yan, Lin
AU  - Yan L
AUID- ORCID: 0000-0002-6002-7542
AD  - Medical College, Yan'an University, Yan'an 716000, People's Republic of China.
FAU - Yan, Yonggang
AU  - Yan Y
AUID- ORCID: 0000-0002-9534-1013
AD  - College of Physical Science and Technology, Sichuan University, Chengdu 610064, 
      People's Republic of China.
FAU - Wang, Cheng
AU  - Wang C
AD  - Medical College, Yan'an University, Yan'an 716000, People's Republic of China.
FAU - Peng, Haitao
AU  - Peng H
AD  - Medical College, Yan'an University, Yan'an 716000, People's Republic of China.
FAU - Fan, Xiaoxia
AU  - Fan X
AUID- ORCID: 0000-0002-7512-3537
AD  - Medical College, Yan'an University, Yan'an 716000, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20240404
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 91D9GV0Z28 (Durapatite)
RN  - H6241UJ22B (Selenium)
RN  - 0 (Amino Acids)
SB  - IM
MH  - Animals
MH  - Rabbits
MH  - *Durapatite/chemistry
MH  - *Selenium
MH  - Amino Acids/chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Bone Regeneration
MH  - Osteogenesis
MH  - Osteoblasts
MH  - Cell Proliferation
OTO - NOTNLM
OT  - bone regeneration
OT  - composites
OT  - cytocompatibility
OT  - osteoconductive
OT  - poly (amino acid)
OT  - selenium-doped hydroxyapatite
EDAT- 2024/03/28 00:44
MHDA- 2024/04/05 06:44
CRDT- 2024/03/27 19:04
PHST- 2023/11/25 00:00 [received]
PHST- 2024/03/26 00:00 [accepted]
PHST- 2024/04/05 06:44 [medline]
PHST- 2024/03/28 00:44 [pubmed]
PHST- 2024/03/27 19:04 [entrez]
AID - 10.1088/1748-605X/ad38ac [doi]
PST - epublish
SO  - Biomed Mater. 2024 Apr 4;19(3). doi: 10.1088/1748-605X/ad38ac.