PMID- 38538265
OWN - NLM
STAT- MEDLINE
DCOM- 20240329
LR  - 20240403
IS  - 2768-6698 (Electronic)
IS  - 2768-6698 (Linking)
VI  - 29
IP  - 3
DP  - 2024 Mar 22
TI  - Identification of Down-Expressed CRNN Associated with Cancer Progression and Poor 
      Prognosis in Laryngeal Squamous Cell Carcinoma.
PG  - 125
LID - 10.31083/j.fbl2903125 [doi]
AB  - BACKGROUND: The prevalence of laryngeal squamous cell carcinoma (LSCC) is 
      increasing, and it poses a significant threat to human health; therefore, 
      identifying specific targets for LSCC remains crucial. METHODS: Bioinformatics 
      analysis was used to compare the different expression genes expressed in LSCC. 
      Immunohistochemical assay and western blotting were used to analysis protein 
      expression. Cell viability was measured by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide)((4,5 Dimethyl 
      thiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide)4,5 Dimethyl 
      thiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) and 5-ethynyl 2'-deoxyuridine 
      (Edu) assay. Flow cytometry was used to measure the cell cycle. Cell migration 
      was measured by wound healing assay and transwell assay. RESULTS: Our analysis 
      revealed 36 upregulated and 65 downregulated differentially expressed genes 
      (DEGs) when comparing LSCC tumors to adjacent tissues, with cornulin (CRNN) 
      identified as a key hub gene connecting these DEGs. We observed a consistent 
      downregulation of CRNN expression in LSCC cell lines and tissues and was 
      associated with poor patient survival and the tumor microenvironment. CRNN 
      overexpression was found to significantly inhibit cell growth, cell cycle 
      progression, migration and invasion, while CRNN knockdown had the opposite 
      effects. Additionally, in vivo experiments demonstrated that CRNN overexpression 
      suppressed tumor growth in nude mice. CONCLUSIONS: CRNN functions as a potential 
      tumor suppressor and regulates important aspects of LSCC, providing valuable 
      insights into the role of CRNN in LSCC pathogenesis and potential for targeted 
      therapeutic interventions.
CI  - (c) 2024 The Author(s). Published by IMR Press.
FAU - Hong, Feilong
AU  - Hong F
AD  - Department of Otolaryngology, Hangzhou Hospital of Zhejiang Medical and Health 
      Group, 310022 Hangzhou, Zhejiang, China.
FAU - Wan, Xuemei
AU  - Wan X
AD  - Department of Otolaryngology, Chengdu First People's Hospital, 610000 Chengdu, 
      Sichuan, China.
FAU - Bai, Yundan
AU  - Bai Y
AD  - Department of Health Management Medical Center, Chengdu First People's Hospital, 
      610000 Chengdu, Sichuan, China.
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Front Biosci (Landmark Ed)
JT  - Frontiers in bioscience (Landmark edition)
JID - 101612996
RN  - 0 (Bromides)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Mice
MH  - Bromides/metabolism
MH  - *Carcinoma, Squamous Cell/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Laryngeal Neoplasms/genetics/metabolism/pathology
MH  - Mice, Nude
MH  - *MicroRNAs/genetics
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics/metabolism/pathology
MH  - Tumor Microenvironment
OTO - NOTNLM
OT  - CRNN
OT  - cancer progression
OT  - laryngeal squamous cell carcinoma
OT  - poor prognosis
COIS- The authors declare no conflict of interest.
EDAT- 2024/03/28 00:44
MHDA- 2024/03/29 06:46
CRDT- 2024/03/27 22:23
PHST- 2023/11/09 00:00 [received]
PHST- 2023/12/22 00:00 [revised]
PHST- 2024/01/03 00:00 [accepted]
PHST- 2024/03/29 06:46 [medline]
PHST- 2024/03/28 00:44 [pubmed]
PHST- 2024/03/27 22:23 [entrez]
AID - S2768-6701(24)01191-2 [pii]
AID - 10.31083/j.fbl2903125 [doi]
PST - ppublish
SO  - Front Biosci (Landmark Ed). 2024 Mar 22;29(3):125. doi: 10.31083/j.fbl2903125.