PMID- 38538755
OWN - NLM
STAT- MEDLINE
DCOM- 20240429
LR  - 20240429
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 73
IP  - 5
DP  - 2024 May
TI  - Bakuchicin alleviates ovalbumin-induced allergic asthma by regulating M2 
      macrophage polarization.
PG  - 725-737
LID - 10.1007/s00011-024-01859-8 [doi]
AB  - OBJECTIVE: Asthma is an airway inflammatory disease caused by activation of 
      numerous immune cells including macrophages. Bakuchicin (BKC) is known to exhibit 
      anti-inflammatory effects and type 2 T helper (Th2) regulation, but has not been 
      investigated for airway inflammation. This study aimed to evaluate the effects of 
      BKC on airway inflammation and demonstrate the mechanisms of macrophage 
      polarization. METHODS: The anti-inflammatory effects were determined using 
      lipopolysaccharide (LPS)-stimulated macrophages. The ovalbumin (OVA)-induced 
      asthma mouse model was used to evaluate the effects of BKC on airway inflammation 
      and Th2 responses. Moreover, the effect of BKC on macrophage polarization was 
      confirmed in bone marrow-derived macrophages (BMDMs) differentiation. RESULTS: 
      BKC suppressed nitric oxide production and expression of pro-inflammatory 
      cytokines by inhibiting signaling pathway in LPS-stimulated macrophages. In an 
      OVA-induced asthma model, BKC treatment alleviated histological changes and mast 
      cell infiltration and reduced the levels of eosinophil peroxidase, 
      beta-hexosaminidase, and immunoglobulin levels. In addition, BKC alleviated Th2 
      responses and M2 macrophage populations in bronchoalveolar fluid. In BMDMs, BKC 
      suppressed IL-4-induced M2 macrophage polarization and the expression of M2 
      markers such as arginase-1 and Fizz-1 through inhibiting sirtuin 2 levels. 
      CONCLUSION: BKC could be a drug candidate for the treatment of allergic asthma.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
FAU - Kim, Yeon-Yong
AU  - Kim YY
AD  - Functional Biomaterial Research Center, Korea Research Institute of Bioscience 
      and Biotechnology (KRIBB), Jeongeup, Republic of Korea.
AD  - Cell and Matrix Research Institute, Department of Pharmacology, School of 
      Medicine, Kyungpook National University, Daegu, Republic of Korea.
FAU - Jeong, Seungwon
AU  - Jeong S
AD  - Functional Biomaterial Research Center, Korea Research Institute of Bioscience 
      and Biotechnology (KRIBB), Jeongeup, Republic of Korea.
FAU - Lee, Seung Woong
AU  - Lee SW
AD  - Functional Biomaterial Research Center, Korea Research Institute of Bioscience 
      and Biotechnology (KRIBB), Jeongeup, Republic of Korea.
FAU - Lee, Seung-Jae
AU  - Lee SJ
AD  - Functional Biomaterial Research Center, Korea Research Institute of Bioscience 
      and Biotechnology (KRIBB), Jeongeup, Republic of Korea.
FAU - Rho, Mun-Chual
AU  - Rho MC
AD  - Functional Biomaterial Research Center, Korea Research Institute of Bioscience 
      and Biotechnology (KRIBB), Jeongeup, Republic of Korea.
FAU - Kim, Sang-Hyun
AU  - Kim SH
AD  - Cell and Matrix Research Institute, Department of Pharmacology, School of 
      Medicine, Kyungpook National University, Daegu, Republic of Korea. 
      shkim72@knu.ac.kr.
FAU - Lee, Soyoung
AU  - Lee S
AD  - Functional Biomaterial Research Center, Korea Research Institute of Bioscience 
      and Biotechnology (KRIBB), Jeongeup, Republic of Korea. sylee@kribb.re.kr.
LA  - eng
GR  - KGM5242322/KRIBB Research Initiative Program/
GR  - 2022M3A9G8082645/National Research Foundation of Korea grants/
PT  - Journal Article
DEP - 20240327
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 9006-59-1 (Ovalbumin)
RN  - 0 (Cytokines)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - *Asthma/drug therapy/chemically induced/immunology
MH  - *Ovalbumin
MH  - *Macrophages/drug effects/immunology
MH  - Mice
MH  - *Mice, Inbred BALB C
MH  - Female
MH  - Cytokines/metabolism
MH  - Nitric Oxide/metabolism
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Anti-Inflammatory Agents/pharmacology/therapeutic use
MH  - Th2 Cells/immunology/drug effects
MH  - Lipopolysaccharides
MH  - Lung/pathology/drug effects/immunology
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - Airway inflammation
OT  - Bakuchicin
OT  - Macrophage polarization
OT  - Macrophages
OT  - Sirtuin 2
EDAT- 2024/03/28 06:45
MHDA- 2024/04/29 13:58
CRDT- 2024/03/28 00:27
PHST- 2023/11/08 00:00 [received]
PHST- 2024/02/02 00:00 [accepted]
PHST- 2023/12/20 00:00 [revised]
PHST- 2024/04/29 13:58 [medline]
PHST- 2024/03/28 06:45 [pubmed]
PHST- 2024/03/28 00:27 [entrez]
AID - 10.1007/s00011-024-01859-8 [pii]
AID - 10.1007/s00011-024-01859-8 [doi]
PST - ppublish
SO  - Inflamm Res. 2024 May;73(5):725-737. doi: 10.1007/s00011-024-01859-8. Epub 2024 
      Mar 27.