PMID- 38541642
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 2075-1729 (Print)
IS  - 2075-1729 (Electronic)
IS  - 2075-1729 (Linking)
VI  - 14
IP  - 3
DP  - 2024 Feb 28
TI  - Unraveling the Impact of Dab1 Gene Silencing on the Expression of Autophagy 
      Markers in Lung Development.
LID - 10.3390/life14030316 [doi]
LID - 316
AB  - The purpose of this study was to evaluate the effects of Dab1 gene silencing on 
      the immunoexpression of light chain 3 beta (Lc3b), glucose regulating protein 78 
      (Grp78), heat shock cognate 71 (Hsc70), mammalian target of rapamycin (mTOR) and 
      lysosomal-associated membrane protein 2A (Lamp2a) in the lung tissue of 
      developing yotari (Dab1(-/-)) and wild-type (wt) mice. The lung epithelium and 
      mesenchyme of the embryos at gestational days E13.5 and E15.5 were examined using 
      immunofluorescence and semi-quantitative methods. In the pulmonary mesenchyme and 
      epithelium, Grp78 and Lc3b of moderate fluorescence reactivity was demonstrated 
      in wt mice for both evaluated time points, while yotari mice exhibited only 
      epithelial reactivity for the same markers. Mild punctate expression of Hsc70 was 
      observed for both genotypes. A significant difference was present when analyzing 
      mTOR expression, where wt mice showed strong perinuclear staining in the 
      epithelium. According to our data, Dab1 gene silencing may result in autophagy 
      abnormalities, which could then cause respiratory system pathologies via 
      defective lung cell degradation by lysosome-dependent cell elimination.
FAU - Rizikalo, Azer
AU  - Rizikalo A
AD  - Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, 
      Bosnia and Herzegovina.
FAU - Maglica, Mirko
AU  - Maglica M
AUID- ORCID: 0000-0001-9264-7597
AD  - Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, 
      Bosnia and Herzegovina.
FAU - Kelam, Nela
AU  - Kelam N
AUID- ORCID: 0000-0002-6529-5474
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, 21000 Split, Croatia.
AD  - Department of Medical Genetics, School of Medicine, University of Mostar, 88000 
      Mostar, Bosnia and Herzegovina.
FAU - Perutina, Ilija
AU  - Perutina I
AD  - Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, 
      Bosnia and Herzegovina.
FAU - Ogorevc, Marin
AU  - Ogorevc M
AUID- ORCID: 0000-0002-3801-1026
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, 21000 Split, Croatia.
FAU - Racetin, Anita
AU  - Racetin A
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, 21000 Split, Croatia.
AD  - Department of Medical Genetics, School of Medicine, University of Mostar, 88000 
      Mostar, Bosnia and Herzegovina.
FAU - Filipovic, Natalija
AU  - Filipovic N
AUID- ORCID: 0000-0002-8943-4109
AD  - Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, 
      Bosnia and Herzegovina.
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, 21000 Split, Croatia.
FAU - Katsuyama, Yu
AU  - Katsuyama Y
AUID- ORCID: 0000-0002-5758-9181
AD  - Department of Anatomy, Shiga University of Medical Science, Otsu 520-2192, Japan.
FAU - Zovko, Zdenka
AU  - Zovko Z
AD  - Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, 
      Bosnia and Herzegovina.
FAU - Miskovic, Josip
AU  - Miskovic J
AD  - Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, 
      Bosnia and Herzegovina.
FAU - Vukojevic, Katarina
AU  - Vukojevic K
AUID- ORCID: 0000-0003-2182-2890
AD  - Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, 
      Bosnia and Herzegovina.
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, 21000 Split, Croatia.
AD  - Department of Medical Genetics, School of Medicine, University of Mostar, 88000 
      Mostar, Bosnia and Herzegovina.
AD  - Center for Translational Research in Biomedicine, University of Split School of 
      Medicine, 21000 Split, Croatia.
LA  - eng
GR  - IP-2022-10-8720/Croatian Science Foundation/
PT  - Journal Article
DEP - 20240228
PL  - Switzerland
TA  - Life (Basel)
JT  - Life (Basel, Switzerland)
JID - 101580444
PMC - PMC10971152
OTO - NOTNLM
OT  - Grp78
OT  - Hsc70
OT  - Lamp2a
OT  - Lc3b
OT  - autophagy
OT  - lung injury
OT  - mTOR
OT  - yotari
COIS- The authors declare no conflict of interest.
EDAT- 2024/03/28 06:45
MHDA- 2024/03/28 06:46
PMCR- 2024/02/28
CRDT- 2024/03/28 01:14
PHST- 2023/12/25 00:00 [received]
PHST- 2024/02/10 00:00 [revised]
PHST- 2024/02/27 00:00 [accepted]
PHST- 2024/03/28 06:46 [medline]
PHST- 2024/03/28 06:45 [pubmed]
PHST- 2024/03/28 01:14 [entrez]
PHST- 2024/02/28 00:00 [pmc-release]
AID - life14030316 [pii]
AID - life-14-00316 [pii]
AID - 10.3390/life14030316 [doi]
PST - epublish
SO  - Life (Basel). 2024 Feb 28;14(3):316. doi: 10.3390/life14030316.