PMID- 38541736
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 2075-1729 (Print)
IS  - 2075-1729 (Electronic)
IS  - 2075-1729 (Linking)
VI  - 14
IP  - 3
DP  - 2024 Mar 20
TI  - Aerobic Exercise Modulates Proteomic Profiles in Gastrocnemius Muscle of db/db 
      Mice, Ameliorating Sarcopenia.
LID - 10.3390/life14030412 [doi]
LID - 412
AB  - Type-2 diabetes mellitus (T2DM)-induced sarcopenia is intertwined with diminished 
      insulin sensitivity and extracellular matrix (ECM) remodeling in skeletal muscle 
      and other organs. Physical activities such as aerobic exercise play a crucial 
      role in regulating blood glucose levels, insulin sensitivity, metabolic pathways, 
      oxidative stress, fibrosis, ECM remodeling, and muscle regeneration by modulating 
      differentially expressed protein (DEP) levels. The objectives of our research 
      were to investigate the effect of six weeks of aerobic exercise on the 
      gastrocnemius and soleus muscle of db/db mice's DEP levels compared to those of 
      sedentary db/db mice. A total of eight db/db mice were divided into two groups (n 
      = 4 per group), namely sedentary mice (SED) and exercise-trained mice (ET), of 
      which the latter were subjected to six weeks of a moderate-intensity aerobic 
      exercise intervention for five days per week. After the exercise intervention, 
      biochemical tests, including analyses of blood glucose and HbA1c levels, were 
      performed. Histological analysis using H & E staining on tissue was performed to 
      compare morphological characters. Gastrocnemius and soleus muscles were dissected 
      and processed for proteomic analysis. Data were provided and analyzed based on 
      the DEPs using the label-free quantification (LFQ) algorithm. Functional 
      enrichment analysis and Ingenuity Pathway Analysis (IPA) were employed as 
      bioinformatics tools to elucidate the molecular mechanisms involved in the DEPs 
      and disease progression. Significantly reduced blood glucose and HbA1c levels and 
      an increased cross-sectional area (CSA) of gastrocnemius muscle fibers were seen 
      in the ET group after the exercise interventions due to upregulations of 
      metabolic pathways. Using proteomics data analysis, we found a significant 
      decrease in COL1A1, COL4A2, ENG, and LAMA4 protein levels in the ET 
      gastrocnemius, showing a significant improvement in fibrosis recovery, ECM 
      remodeling, and muscle regeneration via the downregulation of the TGF-beta signaling 
      pathway. Upregulated metabolic pathways due to ET-regulated DEPs in the 
      gastrocnemius indicated increased glucose metabolism, lipid metabolism, muscle 
      regeneration, and insulin sensitivity, which play a crucial role in muscle 
      regeneration and maintaining blood glucose and lipid levels. No significant 
      changes were observed in the soleus muscle due to the type of exercise and muscle 
      fiber composition. Our research suggests that engaging in six weeks of aerobic 
      exercise may have a positive impact on the recovery of T2DM-induced sarcopenia, 
      which might be a potential candidate for mitigation, prevention, and therapeutic 
      treatment in the future.
FAU - Huang, Yen-Chun
AU  - Huang YC
AD  - Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 
      333325, Taiwan.
AD  - Marker Exploration Corporation, Taipei 112022, Taiwan.
FAU - Sanotra, Monika Renuka
AU  - Sanotra MR
AD  - Marker Exploration Corporation, Taipei 112022, Taiwan.
AD  - Department of Cardiology, Taipei Veterans General Hospital, Taipei 112201, 
      Taiwan.
FAU - Huang, Chi-Chang
AU  - Huang CC
AUID- ORCID: 0000-0003-1446-6787
AD  - Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 
      333325, Taiwan.
AD  - Tajen University, Pingtung 907101, Taiwan.
FAU - Hsu, Yi-Ju
AU  - Hsu YJ
AD  - Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 
      333325, Taiwan.
FAU - Liao, Chen-Chung
AU  - Liao CC
AUID- ORCID: 0000-0002-6634-8355
AD  - Cancer and Immunology Research Center, National Yang Ming Chiao Tung University, 
      Taipei 112304, Taiwan.
LA  - eng
GR  - MOST110-2410-HA49A-514/Ministry of Science and Technology of Taiwan/
PT  - Journal Article
DEP - 20240320
PL  - Switzerland
TA  - Life (Basel)
JT  - Life (Basel, Switzerland)
JID - 101580444
PMC - PMC10971967
OTO - NOTNLM
OT  - ECM remodeling
OT  - T2DM-induced sarcopenia
OT  - aerobic exercise
OT  - db/db mice
OT  - gastrocnemius muscle
OT  - metabolic pathways
OT  - muscle regeneration
OT  - proteomic profiling
COIS- Authors Yen-Chun Huang and Monika Renuka Sanotra were employed by the company 
      Marker Exploration Corporation. The remaining authors declare that the research 
      was conducted in the absence of any commercial or financial relationships that 
      could be construed as a potential conflict of interest.
EDAT- 2024/03/28 06:45
MHDA- 2024/03/28 06:46
PMCR- 2024/03/20
CRDT- 2024/03/28 01:15
PHST- 2024/02/07 00:00 [received]
PHST- 2024/03/15 00:00 [revised]
PHST- 2024/03/15 00:00 [accepted]
PHST- 2024/03/28 06:46 [medline]
PHST- 2024/03/28 06:45 [pubmed]
PHST- 2024/03/28 01:15 [entrez]
PHST- 2024/03/20 00:00 [pmc-release]
AID - life14030412 [pii]
AID - life-14-00412 [pii]
AID - 10.3390/life14030412 [doi]
PST - epublish
SO  - Life (Basel). 2024 Mar 20;14(3):412. doi: 10.3390/life14030412.