PMID- 38542146 OWN - NLM STAT- MEDLINE DCOM- 20240329 LR - 20240330 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 25 IP - 6 DP - 2024 Mar 9 TI - Association between Albumin Alterations and Renal Function in Patients with Type 2 Diabetes Mellitus. LID - 10.3390/ijms25063168 [doi] LID - 3168 AB - Diabetic kidney disease (DKD) is a major cause of morbidity and mortality in individuals with type 2 diabetes mellitus (T2DM). The aim of this study was to investigate whether albumin structural alterations correlate with DKD severity and evaluate whether native and reduced albumin concentrations could complement the diagnosis of DKD. To this end, one hundred and seventeen T2DM patients without (n = 42) and with (n = 75) DKD (DKD I-III upon KDIGO classification) were evaluated; the total albumin concentration (tHA) was quantified by a bromocresol green assay, while structural alterations were profiled via liquid chromatography-high-resolution mass spectrometry (LC-HRMS). The concentrations of native albumin (eHA, effective albumin) and reduced albumin (rHA) were subsequently assessed. The HRMS analyses revealed a reduced relative amount of native albumin in DKD patients along with an increased abundance of altered forms, especially those bearing oxidative modifications. Accordingly, both eHA and rHA values varied during the stages of progressive renal failure, and these alterations were dose-dependently correlated with renal dysfunction. A ROC curve analysis revealed a significantly greater sensitivity and specificity of eHA and rHA than of tHA for diagnosing DKD. Importantly, according to the multivariate logistic regression analysis, the eHA was identified as an independent predictor of DKD. FAU - Nugnes, Marta AU - Nugnes M AD - Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. FAU - Baldassarre, Maurizio AU - Baldassarre M AUID- ORCID: 0000-0001-7865-394X AD - Unit of Semeiotics, Liver and Alcohol-Related Diseases, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Ribichini, Danilo AU - Ribichini D AD - Endocrinology and Diabetes Prevention and Care Department, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Tedesco, Daniele AU - Tedesco D AUID- ORCID: 0000-0003-2585-7791 AD - Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. AD - Institute for Organic Synthesis and Photoreactivity, National Research Council, 40129 Bologna, Italy. FAU - Capelli, Irene AU - Capelli I AD - Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. AD - Nephrology, Dialysis and Renal Transplant Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Vetrano, Daniele AU - Vetrano D AUID- ORCID: 0000-0002-6263-6202 AD - Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. AD - Nephrology, Dialysis and Renal Transplant Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Marchignoli, Francesca AU - Marchignoli F AUID- ORCID: 0000-0002-4862-1274 AD - Unit of Clinical Nutrition, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Brodosi, Lucia AU - Brodosi L AUID- ORCID: 0000-0002-7735-7847 AD - Unit of Clinical Nutrition, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Pompili, Enrico AU - Pompili E AUID- ORCID: 0000-0003-2467-7027 AD - Unit of Semeiotics, Liver and Alcohol-Related Diseases, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. AD - Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. FAU - Petroni, Maria Letizia AU - Petroni ML AUID- ORCID: 0000-0002-7040-6466 AD - Unit of Clinical Nutrition, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - La Manna, Gaetano AU - La Manna G AUID- ORCID: 0000-0001-5473-8551 AD - Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. AD - Nephrology, Dialysis and Renal Transplant Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Marchesini, Giulio AU - Marchesini G AUID- ORCID: 0000-0003-2407-9860 AD - Unit of Clinical Nutrition, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Naldi, Marina AU - Naldi M AUID- ORCID: 0000-0003-4468-2974 AD - Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. AD - Centre for Applied Biomedical Research (CRBA), Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. FAU - Bartolini, Manuela AU - Bartolini M AUID- ORCID: 0000-0002-2890-3856 AD - Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy. LA - eng GR - Almaidea 2017/Italian Ministry for Education, Universities and Research (MIUR) and University of Bologna (RFO funding scheme and Alma Idea 2017 Junior intramural grant)/ PT - Journal Article DEP - 20240309 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *Diabetes Mellitus, Type 2/complications MH - *Diabetic Nephropathies/complications MH - Glomerular Filtration Rate MH - Sensitivity and Specificity MH - Kidney PMC - PMC10970212 OTO - NOTNLM OT - diabetic kidney disease OT - effective albumin OT - high-resolution mass spectrometry OT - oxidative damage OT - reduced albumin OT - structural alterations COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2024/03/28 06:46 MHDA- 2024/03/29 06:46 PMCR- 2024/03/09 CRDT- 2024/03/28 01:17 PHST- 2024/01/10 00:00 [received] PHST- 2024/02/11 00:00 [revised] PHST- 2024/03/07 00:00 [accepted] PHST- 2024/03/29 06:46 [medline] PHST- 2024/03/28 06:46 [pubmed] PHST- 2024/03/28 01:17 [entrez] PHST- 2024/03/09 00:00 [pmc-release] AID - ijms25063168 [pii] AID - ijms-25-03168 [pii] AID - 10.3390/ijms25063168 [doi] PST - epublish SO - Int J Mol Sci. 2024 Mar 9;25(6):3168. doi: 10.3390/ijms25063168.