PMID- 38543564
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 12
IP  - 3
DP  - 2024 Mar 2
TI  - The Molecular Profile of Soil Microbial Communities Inhabiting a Cambrian Host 
      Rock.
LID - 10.3390/microorganisms12030513 [doi]
LID - 513
AB  - The process of soil genesis unfolds as pioneering microbial communities colonize 
      mineral substrates, enriching them with biomolecules released from bedrock. The 
      resultant intricate surface units emerge from a complex interplay among 
      microbiota and plant communities. Under these conditions, host rocks undergo 
      initial weathering through microbial activity, rendering them far from pristine 
      and challenging the quest for biomarkers in ancient sedimentary rocks. In 
      addressing this challenge, a comprehensive analysis utilizing Gas Chromatography 
      Mass Spectrometry (GC-MS) and Time-of-Flight Secondary Ion Mass Spectrometry 
      (ToF-SIMS) was conducted on a 520-Ma-old Cambrian rock. This investigation 
      revealed a diverse molecular assemblage with comprising alkanols, sterols, fatty 
      acids, glycerolipids, wax esters, and nitrogen-bearing compounds. Notably, 
      elevated levels of bacterial C(16), C(18) and C(14) fatty acids, iso and anteiso 
      methyl-branched fatty acids, as well as fungal sterols, long-chained fatty acids, 
      and alcohols, consistently align with a consortium of bacteria and fungi 
      accessing complex organic matter within a soil-type ecosystem. The prominence of 
      bacterial and fungal lipids alongside maturity indicators denotes derivation from 
      heterotrophic activity rather than ancient preservation or marine sources. 
      Moreover, the identification of long-chain (>C22) n-alkanols, 
      even-carbon-numbered long chain (>C20) fatty acids, and campesterol, as well as 
      stigmastanol, provides confirmation of plant residue inputs. Furthermore, 
      findings highlight the ability of contemporary soil microbiota to inhabit rocky 
      substrates actively, requiring strict contamination controls when evaluating 
      ancient molecular biosignatures or extraterrestrial materials collected.
FAU - Huang, Ting
AU  - Huang T
AD  - SKL Lunar and Planetary Sciences, Macau University of Science and Technology, 
      Macau 999078, China.
AD  - CNSA Macau Center for Space Exploration and Science, Macau 999078, China.
FAU - Carrizo, Daniel
AU  - Carrizo D
AUID- ORCID: 0000-0003-1568-4591
AD  - Centro de Astrobiologia (INTA-CSIC), 28850 Madrid, Spain.
FAU - Sanchez-Garcia, Laura
AU  - Sanchez-Garcia L
AD  - Centro de Astrobiologia (INTA-CSIC), 28850 Madrid, Spain.
FAU - Hu, Qitao
AU  - Hu Q
AUID- ORCID: 0000-0002-5748-2421
AD  - SKL Lunar and Planetary Sciences, Macau University of Science and Technology, 
      Macau 999078, China.
AD  - CNSA Macau Center for Space Exploration and Science, Macau 999078, China.
FAU - Angles, Angelica
AU  - Angles A
AD  - Blue Marble Space Institute of Science, Seattle, WA 98104, USA.
FAU - Gomez-Ortiz, David
AU  - Gomez-Ortiz D
AUID- ORCID: 0000-0003-2528-310X
AD  - ESCET-Area de Geologia, Universidad Rey Juan Carlos, 28933 Mostoles, Spain.
FAU - Yu, Liang-Liang
AU  - Yu LL
AD  - SKL Lunar and Planetary Sciences, Macau University of Science and Technology, 
      Macau 999078, China.
AD  - Institute of Science and Technology for Deep Space Exploration, Nanjing 
      University, Suzhou Campus, Suzhou 215163, China.
FAU - Fernandez-Remolar, David C
AU  - Fernandez-Remolar DC
AUID- ORCID: 0000-0003-2409-7444
AD  - SKL Lunar and Planetary Sciences, Macau University of Science and Technology, 
      Macau 999078, China.
AD  - CNSA Macau Center for Space Exploration and Science, Macau 999078, China.
AD  - Carl Sagan Center, The SETI Institute, Mountain View, CA 94043, USA.
LA  - eng
GR  - 2021YFA0716100/Ministry of Science and Technology of the People's Republic of 
      China/
GR  - 2022YFF0504000/Ministry of Science and Technology of the People's Republic of 
      China/
GR  - 0005/2020/A1/Science and Technology Development Fund/
GR  - 0051/2021/A1/Science and Technology Development Fund/
GR  - RYC2018-023943-I/Ministry of Science and Innovation of Spain/
GR  - PID2021-126746NB-I00/Ministry of Science and Innovation of Spain/
GR  - ESP2017-87690-C3-3-R/Ministry of Science and Innovation of Spain/
GR  - PID2022-140180OB-C21/Ministry of Science and Innovation of Spain/
PT  - Journal Article
DEP - 20240302
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10975187
OTO - NOTNLM
OT  - Cambrian host rock
OT  - GC-MS
OT  - ToF-SIMS
OT  - contamination control
OT  - soil microorganisms
COIS- The authors declare no conflict of interest.
EDAT- 2024/03/28 06:44
MHDA- 2024/03/28 06:45
PMCR- 2024/03/02
CRDT- 2024/03/28 01:25
PHST- 2023/12/21 00:00 [received]
PHST- 2024/01/18 00:00 [revised]
PHST- 2024/01/22 00:00 [accepted]
PHST- 2024/03/28 06:45 [medline]
PHST- 2024/03/28 06:44 [pubmed]
PHST- 2024/03/28 01:25 [entrez]
PHST- 2024/03/02 00:00 [pmc-release]
AID - microorganisms12030513 [pii]
AID - microorganisms-12-00513 [pii]
AID - 10.3390/microorganisms12030513 [doi]
PST - epublish
SO  - Microorganisms. 2024 Mar 2;12(3):513. doi: 10.3390/microorganisms12030513.