PMID- 38543614
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 12
IP  - 3
DP  - 2024 Mar 12
TI  - Unveiling Shared Immune Responses in Porcine Alveolar Macrophages during ASFV and 
      PRRSV Infection Using Single-Cell RNA-seq.
LID - 10.3390/microorganisms12030563 [doi]
LID - 563
AB  - African swine fever virus (ASFV) and porcine reproductive and respiratory 
      syndrome virus (PRRSV) infections lead to severe respiratory diseases in pigs, 
      resulting in significant economic losses for the global swine industry. While 
      numerous studies have focused on specific gene functions or pathway activities 
      during infection, an investigation of shared immune responses in porcine alveolar 
      macrophages (PAMs) after ASFV and PRRSV infections was lacking. In this study, we 
      conducted a comparison using two single-cell transcriptomic datasets generated 
      from PAMs under ASFV and PRRSV infection. Pattern recognition receptors (PRRs) 
      RIG-I (DDX58), MDA5 (IFIH1), and LGP2 (DHX58) were identified as particularly 
      recognizing ASFV and PRRSV, triggering cellular defense responses, including the 
      upregulation of four cytokine families (CCL, CXCL, IL, and TNF) and the induction 
      of pyroptosis. Through weighted gene co-expression network analysis and 
      protein-protein interaction analysis, we identified thirteen gene and protein 
      interactions shared by both scRNA-seq analyses, suggesting the ability to inhibit 
      both ASFV and PRRSV viral replication. We discovered six proteins (PARP12, 
      PARP14, HERC5, DDX60, RSAD2, and MNDA) in PAMs as inhibitors of ASFV and PRRSV 
      replication. Collectively, our findings showed detailed characterizations of the 
      immune responses in PAMs during ASFV and PRRSV infections, which may facilitate 
      the treatments of these viral diseases.
FAU - Jiang, Bo
AU  - Jiang B
AUID- ORCID: 0009-0006-1214-0440
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Li, Lu
AU  - Li L
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Wu, Yu
AU  - Wu Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Wang, Xiaoying
AU  - Wang X
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Gao, Ning
AU  - Gao N
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Xu, Zhichao
AU  - Xu Z
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Guo, Chunhe
AU  - Guo C
AUID- ORCID: 0000-0002-7859-1985
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - He, Sheng
AU  - He S
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Zhang, Guihong
AU  - Zhang G
AD  - Research Center for African Swine Fever Prevention and Control, South China 
      Agricultural University, Guangzhou 510642, China.
FAU - Chen, Yaosheng
AU  - Chen Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Liu, Xiaohong
AU  - Liu X
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
FAU - Li, Zhengcao
AU  - Li Z
AUID- ORCID: 0000-0002-7810-9394
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou 510006, China.
AD  - School of Biology, Jiaying University, Meizhou 514015, China.
LA  - eng
GR  - CARS-35/The National Pig Industry Technology System/
GR  - 2022-440000-43010101-9501/The Department of Agriculture of Guangdong Province/
GR  - 32002148/The National Natural Science Foundation of China/
GR  - 2021A1515010770/The Guangdong Basic and Applied Basic Research Foundation/
PT  - Journal Article
DEP - 20240312
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10974629
OTO - NOTNLM
OT  - ASFV
OT  - PAMs
OT  - PRRSV
OT  - immune responses
OT  - pigs
OT  - single-cell RNA sequencing (scRNA-seq)
COIS- We declare that we have no financial and personal relationships with other people 
      or organizations that could inappropriately influence our work. There is no 
      professional or other personal interest of any nature or kind in any product, 
      service, and/or company that could be construed as influencing the position 
      presented in, or the review of, the manuscript entitled.
EDAT- 2024/03/28 06:46
MHDA- 2024/03/28 06:47
PMCR- 2024/03/12
CRDT- 2024/03/28 01:26
PHST- 2024/01/22 00:00 [received]
PHST- 2024/03/01 00:00 [revised]
PHST- 2024/03/07 00:00 [accepted]
PHST- 2024/03/28 06:47 [medline]
PHST- 2024/03/28 06:46 [pubmed]
PHST- 2024/03/28 01:26 [entrez]
PHST- 2024/03/12 00:00 [pmc-release]
AID - microorganisms12030563 [pii]
AID - microorganisms-12-00563 [pii]
AID - 10.3390/microorganisms12030563 [doi]
PST - epublish
SO  - Microorganisms. 2024 Mar 12;12(3):563. doi: 10.3390/microorganisms12030563.