PMID- 3859334
OWN - NLM
STAT- MEDLINE
DCOM- 19850801
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 24
IP  - 7
DP  - 1985 Mar 26
TI  - Phospholipid localization in the plasma membrane of Friend erythroleukemic cells 
      and mouse erythrocytes.
PG  - 1777-83
AB  - The distribution of phospholipids over outer and inner layers of the plasma 
      membranes of Friend erythroleukemic cells (Friend cells) and mature mouse 
      erythrocytes has been determined. The various techniques which have been applied 
      to establish the phospholipid localization include the following: phospholipase 
      A2, phospholipase C, and sphingomyelinase C treatment, fluorescamine labeling of 
      phosphatidylethanolamine, and a phosphatidylcholine transfer protein mediated 
      exchange procedure. The data obtained with these different techniques were found 
      to be in good agreement with each other. Phosphatidylcholine, 
      phosphatidylethanolamine, and phosphatidylinositol were found to be distributed 
      symmetrically over both layers of the plasma membrane of Friend cells. In 
      contrast, sphingomyelin was found to be enriched in the outer layer of the 
      membrane (80-85%), and phosphatidylserine appeared to be present mainly in the 
      inner layer (80-90%). From these results, it was calculated that the outer and 
      inner layers accounted for 46% and 54%, respectively, of the total phospholipid 
      complement of that membrane. Analogous studies on the plasma membrane of mature 
      mouse erythrocytes showed that the transbilayer distribution of the total 
      phospholipid mass appeared to be the same as in the plasma membrane of the Friend 
      cell, namely, 46% and 54% in outer and inner layers, respectively. The outer 
      layer of this membrane contains 57% of the phosphatidylcholine, 20% of the 
      phosphatidylethanolamine, 85% of the sphingomyelin, and 42% of the 
      phosphatidylinositol, and none of the phosphatidylserine was present.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Rawyler, A
AU  - Rawyler A
FAU - van der Schaft, P H
AU  - van der Schaft PH
FAU - Roelofsen, B
AU  - Roelofsen B
FAU - Op den Kamp, J A
AU  - Op den Kamp JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phospholipids)
RN  - 38183-12-9 (Fluorescamine)
RN  - EC 3.1.- (Phospholipases)
SB  - IM
MH  - Animals
MH  - Cell Membrane/metabolism
MH  - Clone Cells/metabolism
MH  - Erythrocyte Membrane/*metabolism
MH  - Fluorescamine
MH  - Friend murine leukemia virus
MH  - Leukemia, Erythroblastic, Acute/*metabolism
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Phosphatidylcholines/metabolism
MH  - Phospholipases/pharmacology
MH  - Phospholipids/blood/*metabolism
EDAT- 1985/03/26 00:00
MHDA- 1985/03/26 00:01
CRDT- 1985/03/26 00:00
PHST- 1985/03/26 00:00 [pubmed]
PHST- 1985/03/26 00:01 [medline]
PHST- 1985/03/26 00:00 [entrez]
AID - 10.1021/bi00328a031 [doi]
PST - ppublish
SO  - Biochemistry. 1985 Mar 26;24(7):1777-83. doi: 10.1021/bi00328a031.