PMID- 3870695
OWN - NLM
STAT- MEDLINE
DCOM- 19880202
LR  - 20190719
IS  - 0007-1145 (Print)
IS  - 0007-1145 (Linking)
VI  - 54
IP  - 3
DP  - 1985 Nov
TI  - Responses to oral methionine supplementation in sheep fed on kale (Brassica 
      oleracea) diets containing S-methyl-L-cysteine sulphoxide.
PG  - 753-61
AB  - 1. Responses to twice-weekly oral supplementation with 4.0 g methionine were 
      measured with lambs (27 kg) grazing kale (Brassica oleracea) for 10 weeks (Expt 
      1). In a second experiment with sheep fed on kale at hourly intervals, rumen 
      fractional outflow rates of CrEDTA and ruthenium Tris(1,10 phenanthroline) 
      markers were measured from the rates of decline in their concentrations. Rumen 
      turnover of S-methyl-L-cysteine sulphoxide (SMCO) and of carbohydrate (CHO) 
      constituents were also measured. The kale fed contained 11.4 g SMCO/kg dry matter 
      and the ratio, readily-fermentable: structural CHO was high at 2.9. 2. Severe 
      haemolytic anaemia, associated with low live-weight gain (LWG), occurred in the 
      lambs during the initial 5 weeks of grazing, due to rumen fermentation of SMCO. 
      Oral methionine supplementation raised plasma concentrations of methionine and 
      cysteine, increased wool growth rate, and increased LWG during the initial 5 
      weeks. Methionine supplementation also increased rumen pool and plasma SMCO 
      concentrations, suggesting reduced rumen SMCO fermentation. 3. In Expt 2, rumen 
      degradation rate of SMCO (1.2/h) was calculated to be twice as fast as that of 
      the most rapidly fermented dietary CHO constituents and eight times faster than 
      the rate of water outflow (0.16/h), thus explaining its virtually complete rumen 
      degradation and toxicity in brassica diets. It was estimated that 1.2 g of each 
      4.0 g methionine administered would have escaped rumen degradation, due to the 
      high rate of water outflow from the rumen. 4. Disappearance rates of CHO 
      constituents from the rumen were as predicted for normal ruminant diets, showing 
      that rumen metabolism of SMCO did not have a depressive effect.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Barry, T N
AU  - Barry TN
AD  - Invermay Agricultural Research Centre, Private Bag, New Zealand.
FAU - Manley, T R
AU  - Manley TR
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Dietary Carbohydrates)
RN  - 4740-94-7 (methiin)
RN  - AE28F7PNPL (Methionine)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - *Animal Feed
MH  - *Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Body Water/metabolism
MH  - Body Weight
MH  - Cysteine/adverse effects/*analogs & derivatives/metabolism
MH  - Dietary Carbohydrates/metabolism
MH  - Fermentation/drug effects
MH  - Methionine/administration & dosage/blood/*pharmacology
MH  - Rumen/metabolism
MH  - Sheep/*physiology
EDAT- 1985/11/01 00:00
MHDA- 1985/11/01 00:01
CRDT- 1985/11/01 00:00
PHST- 1985/11/01 00:00 [pubmed]
PHST- 1985/11/01 00:01 [medline]
PHST- 1985/11/01 00:00 [entrez]
AID - 10.1079/bjn19850161 [doi]
PST - ppublish
SO  - Br J Nutr. 1985 Nov;54(3):753-61. doi: 10.1079/bjn19850161.