PMID- 3871359
OWN - NLM
STAT- MEDLINE
DCOM- 19850228
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 90
IP  - 1
DP  - 1985 Jan
TI  - Characterization of cell lines enhancing IL-2 production by human 
      phytohemagglutinin-stimulated lymphocytes.
PG  - 142-53
AB  - Certain cell lines were found to significantly enhance IL-2 production by 
      phytohemagglutinin-stimulated T lymphocytes, and the mechanisms involved in 
      mediating such an enhancement have been studied. All B-lymphoblastoid cell lines 
      (B-CL) tested had an enhancing capability, even lines which were immature, 
      surface immunoglobulin negative, or negative for EBV-associated antigens or Fc 
      receptors. Furthermore, a B-CL lacking HLA-A, B, and C, antigens as well as a 
      HLA-DR-deficient mutant line enhanced IL-2 production. Autologous B-CL as well as 
      allogeneic lines were able to augment IL-2 production. Cell lines from patients 
      with T-cell acute lymphoblastic leukemia did not stimulate, while more mature, 
      DR-positive T-cell lines did. Although all HLA-DR positive cell lines, regardless 
      of their derivation, provided enhancement, several lines of evidence, including 
      blocking experiments with anti-DR antibodies, indicated that the reaction was not 
      HLA-DR mediated. The enhancing determinant(s) appeared to be cell associated 
      since CL supernatants were ineffective, and it may serve as an important 
      additional signal to the preactivated IL-2-producer T-cell.
FAU - Gramatzki, M
AU  - Gramatzki M
FAU - Bonnard, G D
AU  - Bonnard GD
FAU - Herberman, R B
AU  - Herberman RB
FAU - Strong, D M
AU  - Strong DM
LA  - eng
GR  - Y 01-CP-00319/CP/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-2)
RN  - 0 (Isoantigens)
RN  - 0 (Lymphokines)
RN  - 0 (Phytohemagglutinins)
SB  - IM
MH  - B-Lymphocytes/immunology
MH  - Cell Line
MH  - HLA-DR Antigens
MH  - Histocompatibility Antigens Class II/immunology
MH  - Humans
MH  - Immune Tolerance
MH  - Interleukin-2/*biosynthesis
MH  - Isoantigens/immunology
MH  - *Lymphocyte Activation/*drug effects
MH  - Lymphocytes/*metabolism
MH  - Lymphokines/immunology
MH  - Phytohemagglutinins/*pharmacology
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
AID - 0008-8749(85)90176-5 [pii]
AID - 10.1016/0008-8749(85)90176-5 [doi]
PST - ppublish
SO  - Cell Immunol. 1985 Jan;90(1):142-53. doi: 10.1016/0008-8749(85)90176-5.