PMID- 3874861
OWN - NLM
STAT- MEDLINE
DCOM- 19850829
LR  - 20191101
IS  - 0883-8364 (Print)
IS  - 0883-8364 (Linking)
VI  - 21
IP  - 5
DP  - 1985 May
TI  - The modulation of growth of normal rat liver epithelial cells in calcium-poor 
      medium by epidermal growth factor, phenobarbital, phorbol ester, and retinoic 
      acid.
PG  - 249-53
AB  - The ability of a normal rat liver epithelial cell line with phenotypic 
      characteristics of "oval" cells to grow in calcium-poor medium has been 
      investigated. The growth of these cells could be arrested in medium containing 
      0.03 mM Ca2+, a concentration below which cell necrosis began to occur 24 h 
      postexposure. With increasing calcium concentration, progressive cell 
      proliferation was observed. Epithelial growth factor (EGF) (10 ng/ml) increased 
      the survival and proliferation of cells in calcium-poor medium and the response 
      was inversely correlated with the extracellular calcium concentration. In 
      contrast, phenobarbital (0.2 to 2 mM), 12-O-tetradecanoylphorbol-13-acetate (0.01 
      to 1 microgram/ml), or retinoic acid (0.001 to 0.1 microgram/ml) depressed growth 
      of cells in calcium-poor medium. The results confirm the ability of EGF to lower 
      the calcium requirement for proliferation of normal cells, but such an effect 
      does not seem to be a universal property of tumor promoters.
FAU - Tsao, M S
AU  - Tsao MS
FAU - Smith, J D
AU  - Smith JD
FAU - Grisham, J W
AU  - Grisham JW
LA  - eng
GR  - CA 29323/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - In Vitro Cell Dev Biol
JT  - In vitro cellular & developmental biology : journal of the Tissue Culture 
      Association
JID - 8506951
RN  - 0 (Culture Media)
RN  - 0 (Phorbols)
RN  - 5688UTC01R (Tretinoin)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
RN  - YQE403BP4D (Phenobarbital)
SB  - IM
MH  - Animals
MH  - Calcium
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Culture Media
MH  - Epidermal Growth Factor/*pharmacology
MH  - Epithelial Cells
MH  - Epithelium/drug effects
MH  - Liver/*cytology/drug effects
MH  - Phenobarbital/*pharmacology
MH  - Phorbols/*pharmacology
MH  - Rats
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Time Factors
MH  - Tretinoin/*pharmacology
EDAT- 1985/05/01 00:00
MHDA- 1985/05/01 00:01
CRDT- 1985/05/01 00:00
PHST- 1985/05/01 00:00 [pubmed]
PHST- 1985/05/01 00:01 [medline]
PHST- 1985/05/01 00:00 [entrez]
AID - 10.1007/BF02620937 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol. 1985 May;21(5):249-53. doi: 10.1007/BF02620937.