PMID- 3875406
OWN - NLM
STAT- MEDLINE
DCOM- 19850930
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 45
IP  - 9
DP  - 1985 Sep
TI  - Human colony-stimulating activity-producing tumor: production of very low 
      mouse-active colony-stimulating activity and induction of marked granulocytosis 
      in mice.
PG  - 4144-9
AB  - We reported previously a human lung cancer which induced marked granulocytosis 
      both in the patient and in the tumor-transplanted nude mice (G-1 mice), and whose 
      conditioned media (G-1-T-CM) contained both human-active colony-stimulating 
      activity (h-CSA) (810 colonies/ml) and mouse-active colony-stimulating activity 
      (m-CSA) (530 colonies/ml), suggesting that the CSA produced by the tumor caused 
      granulocytosis both in the patient and in G-1-mice. We reported here another 
      human lung cancer which induced marked granulocytosis both in the patient and in 
      the tumor-transplanted nude mice (G-2-mice). However, in contrast to the tumor 
      reported by us previously, the conditioned media of this tumor (G-2-T-CM) 
      contained only very weak m-CSA (86 colonies/ml), although h-CSA was very strong 
      (7332 colonies/ml). The ratio of m-CSA to h-CSA in G-1-T-CM (0.654) was 55-fold 
      higher than that in G-2-T-CM (0.012). Gel filtration of G-2-T-CM on a Sephadex 
      G-150 column denied the presence of colony-inhibiting activity which inhibited 
      m-CSA in G-2-T-CM. Since the addition of serum obtained from G-2 mice did not 
      enhance m-CSA in G-2-T-CM, it seems unlikely that the G-2 tumor produced an 
      inactive form of m-CSA which was activated in G-2 mouse serum. G-2-T-CM tended to 
      maintain the number of colony-forming units in spleen in mouse bone marrow during 
      liquid cultures, while G-1-T-CM did not. These results may indicate that the G-2 
      tumor produced a humoral factor which has very weak CSA and acts on stem cells 
      rather than on committed progenitors.
FAU - Ikeda, K
AU  - Ikeda K
FAU - Motoyoshi, K
AU  - Motoyoshi K
FAU - Ishizaka, Y
AU  - Ishizaka Y
FAU - Hatake, K
AU  - Hatake K
FAU - Kajigaya, S
AU  - Kajigaya S
FAU - Saito, M
AU  - Saito M
FAU - Miura, Y
AU  - Miura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Culture Media)
SB  - IM
MH  - Animals
MH  - Colony-Stimulating Factors/analysis/*biosynthesis
MH  - Culture Media/analysis
MH  - Granulocytes/*physiology
MH  - Hematopoietic Stem Cells
MH  - Humans
MH  - Leukocytosis/*blood
MH  - Lung Neoplasms/*blood/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Middle Aged
MH  - Neoplasm Transplantation
EDAT- 1985/09/01 00:00
MHDA- 1985/09/01 00:01
CRDT- 1985/09/01 00:00
PHST- 1985/09/01 00:00 [pubmed]
PHST- 1985/09/01 00:01 [medline]
PHST- 1985/09/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1985 Sep;45(9):4144-9.