PMID- 3884247 OWN - NLM STAT- MEDLINE DCOM- 19850501 LR - 20191030 IS - 0174-173X (Print) IS - 0174-173X (Linking) VI - 5 IP - 1 DP - 1985 Jan TI - Immunolocalization of gamma-carboxyglutamic acid-containing proteins in developing molar tooth germs of the rat. PG - 17-22 AB - The localization of dentin gamma-carboxyglutamic acid containing proteins (DGP's) was examined in rat molar tooth germs at various stages of development by immunostaining with the peroxidase-antiperoxidase method. Odontoblasts and their processes stained positive, whereas all other cells were negative. No staining was found in predentin. Dentin stained only after a mild demineralization. The results support the concept that DGP's are deposited by odontoblasts directly into the mineralizing dentin. FAU - Bronckers, A L AU - Bronckers AL FAU - Gay, S AU - Gay S FAU - Dimuzio, M T AU - Dimuzio MT FAU - Butler, W T AU - Butler WT LA - eng GR - DE-02670/DE/NIDCR NIH HHS/United States GR - DE-05092/DE/NIDCR NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - Germany TA - Coll Relat Res JT - Collagen and related research JID - 8102998 RN - 0 (Glutamates) RN - 0 (Minerals) RN - 0 (Proteins) RN - 53445-96-8 (1-Carboxyglutamic Acid) SB - IM MH - 1-Carboxyglutamic Acid/*metabolism MH - Animals MH - Dentin/metabolism MH - Glutamates/*metabolism MH - Histocytochemistry MH - Immunoenzyme Techniques MH - Minerals/metabolism MH - Odontoblasts/metabolism MH - Proteins/*metabolism MH - Rats MH - Rats, Inbred Strains MH - Tooth Germ/growth & development/*metabolism EDAT- 1985/01/01 00:00 MHDA- 1985/01/01 00:01 CRDT- 1985/01/01 00:00 PHST- 1985/01/01 00:00 [pubmed] PHST- 1985/01/01 00:01 [medline] PHST- 1985/01/01 00:00 [entrez] AID - 10.1016/s0174-173x(85)80044-3 [doi] PST - ppublish SO - Coll Relat Res. 1985 Jan;5(1):17-22. doi: 10.1016/s0174-173x(85)80044-3.