PMID- 3886078
OWN - NLM
STAT- MEDLINE
DCOM- 19850614
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 331
IP  - 1
DP  - 1985 Apr 1
TI  - Ultrastructural demonstration of L-glutamate decarboxylase and cysteinesulfinic 
      acid decarboxylase in rat retina by immunocytochemistry.
PG  - 71-80
AB  - The gamma-aminobutyric acid (GABA) synthesizing enzyme, L-glutamate decarboxylase 
      (GAD), and the taurine synthesizing enzyme, cysteinesulfinic acid decarboxylase 
      (CSAD) have been localized in rat retina at the ultrastructural level by indirect 
      immunoelectron microscopy. GAD immunoreactivity (GAD-IR) was seen only in some 
      amacrine cells and their terminals. CSAD immunoreactivity (CSAD-IR) was found in 
      most retinal neuronal types and their processes including photoreceptor cells 
      (rod and cone cells), bipolar cells, amacrine cells and ganglion cells. The 
      GAD-IR positive amacrine terminals have been found to make synaptic contact with 
      other GAD-IR negative bipolar and amacrine terminals, and ganglion cell 
      dendrites. Most of the GAD-IR positive terminals are presynaptic. Occasionally, 
      GAD-IR positive amacrine terminals are postsynaptic to another amacrine terminal 
      or ganglion cell body. In the inner plexiform layer, CSAD-IR positive amacrine 
      terminals also make synaptic contacts with other nerve terminals, similar to that 
      of GAD-IR positive amacrine terminals. In addition, CSAD-IR positive bipolar 
      terminals make synaptic contact with some CSAD-IR positive as well as negative 
      amacrine terminals. Both CSAD-IR positive amacrine and bipolar terminals are 
      mostly presynaptic to other CSAD-IR negative terminals. In the outer plexiform 
      layer, CSAD-IR was found to be associated with synaptic vesicles and the synaptic 
      membrane in certain cone pedicles and rod spherules. It is concluded that only a 
      fraction of amacrine cells in rat retina may use GABA as a neurotransmitter. The 
      presence of CSAD-IR in some amacrine, bipolar, photoreceptor and ganglion cells 
      in rat retina is compatible with the notion that taurine may play some important 
      roles, such as those of neurotransmitter or neuromodulator in mammalian retina.
FAU - Lin, C T
AU  - Lin CT
FAU - Song, G X
AU  - Song GX
FAU - Wu, J Y
AU  - Wu JY
LA  - eng
GR  - EY-05397/EY/NEI NIH HHS/United States
GR  - NS-20922/NS/NINDS NIH HHS/United States
GR  - NS-20978/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 1EQV5MLY3D (Taurine)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.29 (sulfoalanine decarboxylase)
SB  - IM
MH  - Animals
MH  - Carboxy-Lyases/*metabolism
MH  - Glutamate Decarboxylase/*metabolism
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Microscopy, Electron
MH  - Neurons/enzymology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Retina/*enzymology/metabolism/ultrastructure
MH  - Taurine/metabolism
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 1985/04/01 00:00
MHDA- 1985/04/01 00:01
CRDT- 1985/04/01 00:00
PHST- 1985/04/01 00:00 [pubmed]
PHST- 1985/04/01 00:01 [medline]
PHST- 1985/04/01 00:00 [entrez]
AID - 0006-8993(85)90716-4 [pii]
AID - 10.1016/0006-8993(85)90716-4 [doi]
PST - ppublish
SO  - Brain Res. 1985 Apr 1;331(1):71-80. doi: 10.1016/0006-8993(85)90716-4.