PMID- 3887544
OWN - NLM
STAT- MEDLINE
DCOM- 19850529
LR  - 20161123
IS  - 0253-1801 (Print)
IS  - 0253-1801 (Linking)
VI  - 23
IP  - 1
DP  - 1985 Jan-Mar
TI  - Radioreceptorassay of insulin using human erythrocytes. I. Methodological 
      aspects.
PG  - 29-38
AB  - It is known that human erythrocytes have specific receptors for insulin. This 
      works describes a radioreceptor assay (RRA) suitable for the determination of the 
      insulin binding to the surface receptors of erythrocytes. The erythrocytes were 
      isolated from heparinated blood collected in the morning after overnight fasting. 
      In the incubation mixture are 4-5 X 10(9) cells/ml, labelled porcine insulin and 
      nonlabeled (cold) insulin in serial concentrations, range 10-10(5) ng/ml. 
      Incubation (150 min. at +15 degrees C) is ended by suspending the incubation 
      mixture in cold buffer. After washing, the tracer insulin bound to erythrocytes 
      is measured on a gamma counter and the percentage of the specific binding is 
      calculated against the total radioactivity determined prior to the washing steps. 
      By this procedure, the maximal binding of 125I-Insulin was 10.55 +/- 0.78% (mean 
      +/- SD) in five normal volunteers and 6.79 +/- 1.77 (mean +/- SD) in five obese 
      nondiabetic patients, having an enhanced insulin response in the oral glucose 
      tolerance test. The slight hemolysis that happened accidentally in some tubes 
      after incubation caused rude degradation of the tracer, the binding being 
      decreased at last by 25%. The method described (according to Gambhir K. K. et al 
      1977 slightly modified) is specific and thus suitable for estimating the 
      prognosis of obese children, patients with acute and chronic hepatitis and also 
      for the study of the role of erythrocytes in insulin metabolism and possibly in 
      the metabolism of other hormones.
FAU - Simionescu, L
AU  - Simionescu L
FAU - Aman, E
AU  - Aman E
FAU - Zamfir-Grigorescu, D
AU  - Zamfir-Grigorescu D
FAU - Dimitriu, V
AU  - Dimitriu V
FAU - Oniciu, C D
AU  - Oniciu CD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Romania
TA  - Endocrinologie
JT  - Endocrinologie
JID - 7509386
RN  - 0 (Insulin)
RN  - 0 (Insulin, Regular, Pork)
RN  - AVT680JB39 (insulin, neutral)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Erythrocytes/*ultrastructure
MH  - Female
MH  - Glucose Tolerance Test
MH  - Humans
MH  - Insulin/blood/isolation & purification/*metabolism
MH  - Insulin, Regular, Pork
MH  - Male
MH  - Obesity/metabolism
MH  - Radioimmunoassay
MH  - Radioligand Assay
MH  - Receptor, Insulin/*metabolism
MH  - Swine
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
PST - ppublish
SO  - Endocrinologie. 1985 Jan-Mar;23(1):29-38.