PMID- 3890630
OWN - NLM
STAT- MEDLINE
DCOM- 19850722
LR  - 20061115
IS  - 0002-9645 (Print)
IS  - 0002-9645 (Linking)
VI  - 46
IP  - 5
DP  - 1985 May
TI  - Effect of in vitro inoculation of bovine respiratory syncytial virus on bovine 
      pulmonary alveolar macrophage function.
PG  - 1098-103
AB  - Viruses may predispose the respiratory tract to the development of secondary 
      bacterial pneumonia by impairing functions of alveolar macrophages. The effects 
      of bovine respiratory syncytial virus (BRSV) on selected functions of bovine 
      pulmonary alveolar macrophages (PAM) were examined in vitro. Alveolar macrophages 
      were obtained from nonsedated cattle, using a polypropylene tube passed 
      intranasally into the lung. The PAM lavaged from the lung were allowed to adhere 
      to glass coverslips or plastic tissue culture plates, and were exposed to BRSV 
      for 2 hours. Control and BRSV-inoculated PAM were compared at intervals over a 
      72-hour period for their abilities to phagocytize and kill Staphylococcus 
      epidermidis, rosette with and phagocytize antibody-coated sheep RBC (SRBC), 
      phagocytize latex particles, and influence lysosomal enzyme activity. Challenge 
      exposure with BRSV did not affect the ability of PAM to adhere and did not affect 
      cell viability. There were numerical differences between control and 
      BRSV-inoculated cell populations in phagocytosis and killing of S epidermidis, 
      but these were not significant (P greater than 0.05). There was less than 5% 
      difference in the abilities of control and BRSV-challenged PAM to phagocytize 
      latex beads. When Fc-receptor-mediated phagocytosis of antibody-coated SRBC was 
      compared with controls, BRSV-challenged PAM had significantly (P less than 0.05) 
      impaired phagocytic function, which was maximal 72 hours after BRSV inoculation; 
      the phagocytic impairment occurred in spite of normal Fc-receptor function, as 
      determined by rosetting with antibody-coated SRBC.(ABSTRACT TRUNCATED AT 250 
      WORDS)
FAU - Trigo, E
AU  - Trigo E
FAU - Liggitt, H D
AU  - Liggitt HD
FAU - Evermann, J F
AU  - Evermann JF
FAU - Breeze, R G
AU  - Breeze RG
FAU - Huston, L Y
AU  - Huston LY
FAU - Silflow, R
AU  - Silflow R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
RN  - EC 3.1.3.2 (Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/blood
MH  - Animals
MH  - Cattle/*immunology
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Fluorescent Antibody Technique
MH  - Macrophages/enzymology/*immunology/microbiology
MH  - *Phagocytosis
MH  - Pulmonary Alveoli/cytology
MH  - Respiratory Syncytial Viruses/growth & development/*immunology
MH  - Staphylococcus epidermidis/*immunology
MH  - Time Factors
EDAT- 1985/05/01 00:00
MHDA- 1985/05/01 00:01
CRDT- 1985/05/01 00:00
PHST- 1985/05/01 00:00 [pubmed]
PHST- 1985/05/01 00:01 [medline]
PHST- 1985/05/01 00:00 [entrez]
PST - ppublish
SO  - Am J Vet Res. 1985 May;46(5):1098-103.