PMID- 3891969
OWN - NLM
STAT- MEDLINE
DCOM- 19850820
LR  - 20171116
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 234
IP  - 1
DP  - 1985 Jul
TI  - alpha-Glucosidase-albumin conjugates: effect of chronic administration in mice.
PG  - 250-4
AB  - Enzyme albumin conjugates have been proposed as a means of increasing the 
      efficacy of enzyme use in vivo and decreasing immune response to the enzyme. 
      Particulate drug carriers, however, have a pronounced tendency to localize in the 
      mononuclear phagocyte (reticuloendothelial) system. We have examined in mice the 
      effect on phagocytic index, tissue distribution and organ size of continued 
      administration of conjugates of alpha-glucosidase with either homologous or 
      heterologous albumin. Mice received 10 X 2-mg injections of bovine serum albumin 
      (BSA) or mouse serum albumin (MSA), either free, polymerized or conjugated with 
      alpha-glucosidase. Experiments involving BSA had to be terminated before the end 
      of the experiment because of anaphylaxis, but these reactions were less severe to 
      the polymerized albumin than to free albumin. Free BSA, BSA polymer and 
      BSA-enzyme conjugates all caused a decrease in phagocytic index after six 
      injections. Mice receiving MSA showed no evidence of anaphylaxis, but mice 
      receiving six or more injections of free MSA, MSA polymer or MSA-enzyme conjugate 
      had significantly decreased phagocytic indices as compared to controls. 
      Phagocytic indices had returned to normal by 7 days after the final injection. 
      Tissue distribution of 125-I-labeled albumin preparations was determined in 
      either naive or chronically injected mice.
FAU - Allen, T M
AU  - Allen TM
FAU - Murray, L
AU  - Murray L
FAU - Bhardwaj, D
AU  - Bhardwaj D
FAU - Poznansky, M J
AU  - Poznansky MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Liposomes)
RN  - 0 (Serum Albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - EC 3.2.1.- (Glucosidases)
RN  - EC 3.2.1.- (alpha-1,4-glucosidase-albumin polymer)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - Animals
MH  - Glucosidases/*pharmacology
MH  - Iodine Radioisotopes
MH  - Liposomes/administration & dosage
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Mononuclear Phagocyte System/drug effects
MH  - Phagocytosis/drug effects
MH  - Serum Albumin/metabolism/*pharmacology
MH  - Serum Albumin, Bovine/pharmacology
MH  - Tissue Distribution
MH  - alpha-Glucosidases/metabolism/*pharmacology
EDAT- 1985/07/01 00:00
MHDA- 1985/07/01 00:01
CRDT- 1985/07/01 00:00
PHST- 1985/07/01 00:00 [pubmed]
PHST- 1985/07/01 00:01 [medline]
PHST- 1985/07/01 00:00 [entrez]
PST - ppublish
SO  - J Pharmacol Exp Ther. 1985 Jul;234(1):250-4.