PMID- 3897800 OWN - NLM STAT- MEDLINE DCOM- 19851001 LR - 20190820 IS - 0385-5600 (Print) IS - 0385-5600 (Linking) VI - 29 IP - 5 DP - 1985 TI - Nucleotide sequence of the replication region of plasmid R401 and its incompatibility function. PG - 383-93 AB - The plasmids R401 and Rtsl belong to the same incompatibility group, IncT. The nucleotide sequence of the basic replicon of R401 consisting of 1,857 base pairs was determined and compared with that of mini-Rtsl previously reported. The mini-R401 was found to be composed of two clusters of direct repeated sequences flanking a large open reading frame that could encode a 33,000 Mr protein (RepA protein) consisting of 288 amino acids. This structure of mini-R401 is quite similar to that of mini-Rtsl. Furthermore, the nucleotide sequence of mini-R401 is identical to that of mini-Rtsl except for eleven nucleotides; three are located near the carboxyl terminus portion of the RepA coding region (repA) and four are in the repeated sequences (incI) located downstream from repA. Incompatibility study showed that mini-R401 plasmid coexisted stably with the cloned incI repeats of mini-Rtsl, suggesting that mini-R401 RepA protein binds to incI repeats of mini-Rtsl less efficiently than does mini-Rtsl RepA protein. FAU - Tabuchi, A AU - Tabuchi A LA - eng PT - Journal Article PL - Australia TA - Microbiol Immunol JT - Microbiology and immunology JID - 7703966 RN - 0 (Bacterial Proteins) RN - 0 (DNA, Bacterial) SB - IM MH - Bacterial Proteins/genetics/metabolism MH - Base Sequence MH - Cloning, Molecular MH - DNA Replication MH - DNA, Bacterial MH - Escherichia coli/genetics MH - *R Factors MH - Repetitive Sequences, Nucleic Acid MH - *Replicon MH - Transformation, Bacterial EDAT- 1985/01/01 00:00 MHDA- 1985/01/01 00:01 CRDT- 1985/01/01 00:00 PHST- 1985/01/01 00:00 [pubmed] PHST- 1985/01/01 00:01 [medline] PHST- 1985/01/01 00:00 [entrez] AID - 10.1111/j.1348-0421.1985.tb00839.x [doi] PST - ppublish SO - Microbiol Immunol. 1985;29(5):383-93. doi: 10.1111/j.1348-0421.1985.tb00839.x.