PMID- 3922954
OWN - NLM
STAT- MEDLINE
DCOM- 19850719
LR  - 20210526
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 162
IP  - 3
DP  - 1985 Jun
TI  - Cloning of genes specifying carbohydrate catabolism in Pseudomonas aeruginosa and 
      Pseudomonas putida.
PG  - 865-71
AB  - A 6.0-kilobase EcoRI fragment of the Pseudomonas aeruginosa PAO chromosome 
      containing a cluster of genes specifying carbohydrate catabolism was cloned into 
      the multicopy plasmid pRO1769. The vector contains a unique EcoRI site for 
      cloning within a streptomycin resistance determinant and a selectable gene 
      encoding gentamicin resistance. Mutants of P. aeruginosa PAO transformed with the 
      chimeric plasmid pRO1816 regained the ability to grow on glucose, and the 
      following deficiencies in enzyme or transport activities corresponding to the 
      specific mutations were complemented: glcT1, glucose transport and periplasmic 
      glucose-binding protein; glcK1, glucokinase; and edd-1, 6-phosphogluconate 
      dehydratase. Two other carbohydrate catabolic markers that are cotransducible 
      with glcT1 and edd-1 were not complemented by plasmid pRO1816: zwf-1, 
      glucose-6-phosphate dehydrogenase; and eda-9001, 
      2-keto-3-deoxy-6-phosphogluconate aldolase. However, all five of these normally 
      inducible activities were expressed at markedly elevated basal levels when 
      transformed cells of prototrophic strain PAO1 were grown without carbohydrate 
      inducer. Vector plasmid pRO1769 had no effect on the expression of these 
      activities in transformed mutant or wild-type cells. Thus, the chromosomal insert 
      in pRO1816 contains the edd and glcK structural genes, at least one gene (glcT) 
      that is essential for expression of the glucose active transport system, and 
      other loci that regulate the expression of the five clustered carbohydrate 
      catabolic genes. The insert in pRO1816 also complemented the edd-1 mutation in a 
      glucose-negative Pseudomonas putida mutant but not the eda-1 defect in another 
      mutant. Moreover, pRO1816 caused the expression of high specific activities of 
      glucokinase, an enzyme that is naturally lacking in these strains of Pseudomonas 
      putida.
FAU - Cuskey, S M
AU  - Cuskey SM
FAU - Wolff, J A
AU  - Wolff JA
FAU - Phibbs, P V Jr
AU  - Phibbs PV Jr
FAU - Olsen, R H
AU  - Olsen RH
LA  - eng
GR  - AI07086/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Carrier Proteins)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - EC 1.1.1.43 (Phosphogluconate Dehydrogenase)
RN  - EC 4.1.2.- (Aldehyde-Lyases)
RN  - EC 4.1.2.14 (phospho-2-keto-3-deoxy-gluconate aldolase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Aldehyde-Lyases/analysis
MH  - Biological Transport
MH  - *Carbohydrate Metabolism
MH  - Carrier Proteins/biosynthesis
MH  - Cloning, Molecular
MH  - *Genes, Bacterial
MH  - Glucose/metabolism
MH  - Monosaccharide Transport Proteins
MH  - Mutation
MH  - Phosphogluconate Dehydrogenase/analysis
MH  - Plasmids
MH  - Pseudomonas/*genetics/metabolism
MH  - Pseudomonas aeruginosa/*genetics
PMC - PMC215855
EDAT- 1985/06/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1985/06/01
CRDT- 1985/06/01 00:00
PHST- 1985/06/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1985/06/01 00:00 [entrez]
PHST- 1985/06/01 00:00 [pmc-release]
AID - 10.1128/jb.162.3.865-871.1985 [doi]
PST - ppublish
SO  - J Bacteriol. 1985 Jun;162(3):865-71. doi: 10.1128/jb.162.3.865-871.1985.