PMID- 3926877
OWN - NLM
STAT- MEDLINE
DCOM- 19850916
LR  - 20190820
IS  - 0263-6352 (Print)
IS  - 0263-6352 (Linking)
VI  - 3
IP  - 2
DP  - 1985 Apr
TI  - Defective renal adenylate cyclase response to prostaglandin E2 in spontaneously 
      hypertensive rats.
PG  - 159-65
AB  - We activated three known components of the adenylate cyclase system in renal 
      membranes from spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. The 
      basal adenylate cyclase activity and responses to plasma membrane receptor 
      activation by parathyroid hormone, isoproterenol and vasopressin were not 
      different between the two strains. The response to prostaglandin E2 (PGE2), 
      however, was less in the SHR than in the WKY at five, (P less than 0.05), 12 (P 
      less than 0.01) and 16 (P less than 0.01) weeks of age. Activation of either the 
      guanosine-5'-triphosphate (GTP) binding regulatory protein (N) with sodium 
      fluoride (NaF) and guanyl-5'-yl-imidodiphosphate [Gpp(NH)p], or the catalytic 
      unit with manganese chloride (MnCl2) or forskolin were not different between the 
      two groups. When the medullary and cortical plasma membrane adenylate cyclase 
      responses were studied separately, the observed decreased response to PGE2 (of 
      SHR) was found to be entirely in the cortex. Also, the NaF response was reduced 
      in the cortical region of the 12-week-old rats, a finding suggesting a 
      possibility of a post receptor defect. These results show that there is a 
      defective renal adenylate cyclase response specific to prostaglandin E2 in SHR. 
      This defect could be related to the development of hypertension, by changing the 
      natriuretic and/or renal vasodilating effects of these prostaglandins.
FAU - Umemura, S
AU  - Umemura S
FAU - Smyth, D D
AU  - Smyth DD
FAU - Pettinger, W A
AU  - Pettinger WA
LA  - eng
GR  - 1-R01-HL30339-01/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Hypertens
JT  - Journal of hypertension
JID - 8306882
RN  - 0 (Prostaglandins E)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Dinoprostone
MH  - Enzyme Activation/drug effects
MH  - GTP-Binding Proteins/metabolism
MH  - Kidney/*enzymology
MH  - Kidney Cortex/enzymology
MH  - Male
MH  - Prostaglandins E/*pharmacology
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
EDAT- 1985/04/01 00:00
MHDA- 1985/04/01 00:01
CRDT- 1985/04/01 00:00
PHST- 1985/04/01 00:00 [pubmed]
PHST- 1985/04/01 00:01 [medline]
PHST- 1985/04/01 00:00 [entrez]
AID - 10.1097/00004872-198504000-00009 [doi]
PST - ppublish
SO  - J Hypertens. 1985 Apr;3(2):159-65. doi: 10.1097/00004872-198504000-00009.