PMID- 3927909
OWN - NLM
STAT- MEDLINE
DCOM- 19850916
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 130
IP  - 2
DP  - 1985 Jul 31
TI  - Time-dependent inhibition of the cyclooxygenase pathway by 
      12-hydroperoxy-5,8,10,14-eicosatetraenoic acid.
PG  - 781-5
AB  - We examined effects of small dose (1 microM or less) of exogenous 
      12-hydroperoxy-5,8,10,14-eicosatetraenoic acid (12-HPETE) on the formation of 
      cyclooxygenase products from exogenous arachidonic acid (AA) in washed human 
      platelets. With a simultaneous addition of AA, 12-HPETE did not affect the 
      formation of thromboxane (TX)B2 and 12-hydroxy-5,8,10-heptadecatrienoic acid 
      (HHT). However, by being preincubated with platelets before an addition of AA, 
      0.1 microM or greater of 12-HPETE inhibited the formation of TXB2 and HHT 
      dose-dependently. In addition, the inhibitory effect of 12-HPETE increased as the 
      preincubation time was prolonged. These results suggest that 12-HPETE is a strong 
      inhibitor for the cyclooxygenase pathway.
FAU - Hashimoto, Y
AU  - Hashimoto Y
FAU - Naito, C
AU  - Naito C
FAU - Teramoto, T
AU  - Teramoto T
FAU - Kato, H
AU  - Kato H
FAU - Kinoshita, M
AU  - Kinoshita M
FAU - Kawamura, M
AU  - Kawamura M
FAU - Hayashi, H
AU  - Hayashi H
FAU - Oka, H
AU  - Oka H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Arachidonic Acids)
RN  - 0 (Cyclooxygenase Inhibitors)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (Leukotrienes)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 54397-85-2 (Thromboxane B2)
RN  - 59985-28-3 (12-Hydroxy-5,8,10,14-eicosatetraenoic Acid)
RN  - 67675-13-2 (12-HPETE)
SB  - IM
MH  - 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
MH  - Arachidonic Acid
MH  - Arachidonic Acids/metabolism/*pharmacology
MH  - Blood Platelets/drug effects/*enzymology
MH  - *Cyclooxygenase Inhibitors
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Hydroxyeicosatetraenoic Acids/biosynthesis
MH  - *Leukotrienes
MH  - Thromboxane B2/biosynthesis
MH  - Time Factors
EDAT- 1985/07/31 00:00
MHDA- 1985/07/31 00:01
CRDT- 1985/07/31 00:00
PHST- 1985/07/31 00:00 [pubmed]
PHST- 1985/07/31 00:01 [medline]
PHST- 1985/07/31 00:00 [entrez]
AID - 0006-291X(85)90484-X [pii]
AID - 10.1016/0006-291x(85)90484-x [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1985 Jul 31;130(2):781-5. doi: 
      10.1016/0006-291x(85)90484-x.