PMID- 3928668 OWN - NLM STAT- MEDLINE DCOM- 19850930 LR - 20190629 VI - 327 DP - 1985 Jun 26 TI - Isolation and quantitation of serum IgE levels by high-performance immunoaffinity chromatography. PG - 205-11 AB - High-performance immunoaffinity chromatography on monoclonal antibodies coupled to protein A-coated glass beads is a method for the rapid isolation and quantitation of immunoglobulin E (IgE) from the serum of both adult and pediatric patients. The technique is as sensitive as most immunoassays but takes less than an hour to perform. In addition to measuring total IgE in both plasma and serum, the technique provides biologically active, affinity-isolated IgE, which can be used for other clinical and research studies. FAU - Phillips, T M AU - Phillips TM FAU - More, N S AU - More NS FAU - Queen, W D AU - Queen WD FAU - Thompson, A M AU - Thompson AM LA - eng PT - Journal Article PL - Netherlands TA - J Chromatogr JT - Journal of chromatography JID - 0427043 RN - 0 (Staphylococcal Protein A) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Animals MH - Chromatography, Affinity MH - Glass MH - Humans MH - Immunodiffusion MH - Immunoglobulin E/*isolation & purification MH - Mice MH - Mice, Inbred BALB C MH - Staphylococcal Protein A EDAT- 1985/06/26 00:00 MHDA- 1985/06/26 00:01 CRDT- 1985/06/26 00:00 PHST- 1985/06/26 00:00 [pubmed] PHST- 1985/06/26 00:01 [medline] PHST- 1985/06/26 00:00 [entrez] AID - 10.1016/s0021-9673(01)81650-2 [doi] PST - ppublish SO - J Chromatogr. 1985 Jun 26;327:205-11. doi: 10.1016/s0021-9673(01)81650-2.