PMID- 3932580
OWN - NLM
STAT- MEDLINE
DCOM- 19851211
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 162
IP  - 5
DP  - 1985 Nov 1
TI  - Oxidative degradation of leukotriene C4 by human monocytes and monocyte-derived 
      macrophages.
PG  - 1634-44
AB  - Freshly isolated 2-h adherent normal human monocytes, when stimulated, degrade 
      added leukotriene C4 (LTC4) by a myeloperoxidase (MPO) and H2O2-dependent 
      mechanism. Among the stimuli effective in this regard are phorbol myristate 
      acetate (PMA), the calcium ionophore A23187, opsonized zymosan, and 
      N-formyl-methionine-leucine-phenylalanine (FMLP) when combined with cytochalasin 
      B. The predominant products formed are the all-trans isomers of LTB4, 5-(S), 
      12-(R)-6-trans-LTB4 and 5-(S),12-(S)-6-trans-LTB4. Degradation is inhibited by 
      azide and catalase, but not by superoxide dismutase. LTC4 degradation does not 
      occur when MPO-deficient monocytes are used, unless MPO is added. Stimulated 
      monocytes from patients with chronic granulomatous disease also are unable to 
      degrade LTC4 under these conditions. Normal monocytes maintained in culture lose 
      their ability to degrade LTC4. The addition of MPO to monocyte-derived 
      macrophages increases degradation, particularly when the monolayers are 
      pretreated with gamma-interferon. The oxidative degradation of LTC4 is a capacity 
      shared by neutrophils, eosinophils, and mononuclear phagocytes, and may be an 
      important mechanism for the modulation of leukotriene activity in inflammatory 
      lesions.
FAU - Neill, M A
AU  - Neill MA
FAU - Henderson, W R
AU  - Henderson WR
FAU - Klebanoff, S J
AU  - Klebanoff SJ
LA  - eng
GR  - A107763/PHS HHS/United States
GR  - A117758/PHS HHS/United States
GR  - HL30542/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (SRS-A)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Calcimycin/pharmacology
MH  - Cells, Cultured
MH  - Humans
MH  - Kinetics
MH  - Macrophages/*immunology
MH  - Monocytes/drug effects/*immunology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Oxidation-Reduction
MH  - SRS-A/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
PMC - PMC2187916
EDAT- 1985/11/01 00:00
MHDA- 1985/11/01 00:01
PMCR- 1986/05/01
CRDT- 1985/11/01 00:00
PHST- 1985/11/01 00:00 [pubmed]
PHST- 1985/11/01 00:01 [medline]
PHST- 1985/11/01 00:00 [entrez]
PHST- 1986/05/01 00:00 [pmc-release]
AID - 86035944 [pii]
AID - 10.1084/jem.162.5.1634 [doi]
PST - ppublish
SO  - J Exp Med. 1985 Nov 1;162(5):1634-44. doi: 10.1084/jem.162.5.1634.