PMID- 3933899
OWN - NLM
STAT- MEDLINE
DCOM- 19860116
LR  - 20191022
IS  - 0147-9571 (Print)
IS  - 0147-9571 (Linking)
VI  - 8
IP  - 1
DP  - 1985
TI  - Interaction of bovine immunoglobulin gamma 2 (IgG2) with staphylococcal protein 
      A: evidence for IgG2a and IgG2b sub-subclasses in the bovine.
PG  - 1-8
AB  - The reactivity of bovine IgG with protein A is confusing with respect to which of 
      the bovine IgG class and subclasses are reactive. We have, therefore, re-examined 
      the interaction of bovine immunoglobulins with protein A. The results presented 
      in this paper indicated that at pH 8.0 protein A binds only immunoglobulin of the 
      IgG2 subclass. The bound IgG2 can be readily recovered from an immobilized 
      protein A column at pH 5.0. Furthermore, the antigenic IgG2 eluted demonstrated 
      two charged species which could readily be separated by ion-exchange 
      chromatography. These results indicate that IgG2 in the bovine exists in two 
      sub-subclasses, IgG2a and IgG2b. The two sub-subclasses of IgG2 could be rapidly 
      isolated with a good yield in two-steps namely protein A affinity chromatography 
      followed by ion exchange chromatography.
FAU - Lawman, M J
AU  - Lawman MJ
FAU - Joiner, S
AU  - Joiner S
FAU - Gauntlett, D R
AU  - Gauntlett DR
FAU - Boyle, M D
AU  - Boyle MD
LA  - eng
GR  - AI 20445/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Comp Immunol Microbiol Infect Dis
JT  - Comparative immunology, microbiology and infectious diseases
JID - 7808924
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Staphylococcal Protein A)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/classification/immunology/isolation & purification
MH  - Brucella abortus/immunology
MH  - Cattle/*immunology
MH  - Chromatography, DEAE-Cellulose
MH  - Immunoglobulin G/classification/*immunology/isolation & purification
MH  - Protein Binding
MH  - Staphylococcal Protein A/*immunology
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
AID - 0147-9571(85)90048-7 [pii]
AID - 10.1016/0147-9571(85)90048-7 [doi]
PST - ppublish
SO  - Comp Immunol Microbiol Infect Dis. 1985;8(1):1-8. doi: 
      10.1016/0147-9571(85)90048-7.