PMID- 3934083
OWN - NLM
STAT- MEDLINE
DCOM- 19851226
LR  - 20190816
IS  - 0020-5915 (Print)
IS  - 0020-5915 (Linking)
VI  - 78
IP  - 4
DP  - 1985
TI  - Fragments of IgE antibodies in human feces.
PG  - 358-63
AB  - The gel filtration profile of immunoglobulin E (IgE) in extracts of feces from 2 
      children was compared with IgE myeloma protein which had been exposed to 
      proteolytic digestion by chymotrypsin. The peak of the chymotrypsin-digested IgE 
      myeloma protein was found to be similar to that of fecal IgE after an elution 
      volume between those of albumin and myoglobin, corresponding to a molecular 
      weight of approximately 40,000 daltons. In the fractions where the peak of fecal 
      IgE was found, no evidence for the presence of specific IgE antibodies (measured 
      by RAST) could be detected. Fecal IgE could be purified by using an immunosorbent 
      column to which rabbit antihuman IgE was coupled. Sufficient amounts of fecal IgE 
      could thus be obtained and used in autoradiographic experiments. The 
      IgE-containing fractions could also be detected with 125I-labelled second 
      antibodies to visualize the IgE precipitates.
FAU - Kolmannskog, S
AU  - Kolmannskog S
FAU - Marhaug, G
AU  - Marhaug G
FAU - Haneberg, B
AU  - Haneberg B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Int Arch Allergy Appl Immunol
JT  - International archives of allergy and applied immunology
JID - 0404561
RN  - 0 (Antibodies)
RN  - 0 (Myeloma Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies/immunology
MH  - Child
MH  - Chromatography, Affinity
MH  - Chromatography, Gel
MH  - Dialysis
MH  - Feces/*immunology
MH  - Female
MH  - Humans
MH  - Immunodiffusion
MH  - Immunoglobulin E/*analysis
MH  - Male
MH  - Myeloma Proteins/analysis
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
AID - 10.1159/000233913 [doi]
PST - ppublish
SO  - Int Arch Allergy Appl Immunol. 1985;78(4):358-63. doi: 10.1159/000233913.