PMID- 3949889
OWN - NLM
STAT- MEDLINE
DCOM- 19860403
LR  - 20071114
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 126
IP  - 3
DP  - 1986 Mar
TI  - Effect of amino acid levels on matrix vesicle formation by epiphyseal growth 
      plate chondrocytes in primary culture.
PG  - 399-406
AB  - The effect of varying the amino acid concentrations of the culture medium on 
      matrix vesicle formation was studied in primary cultures of chicken epiphyseal 
      growth plate chondrocytes grown in Dulbecco's modified Eagle's medium (DME) 
      supplemented with 10% fetal bovine serum (FBS). Decreasing the levels of free 
      amino acids in the culture medium to levels of one-half, one quarter, and one 
      eighth of the values normally present in DME caused a progressive decline in 
      matrix vesicle (MV) formation. Increasing the level in the culture medium of 
      those amino acids that are enriched in extracellular fluid (ECF) of growth plate 
      cartilage significantly increased formation of matrix vesicles (MV), as assayed 
      by the alkaline phosphatase (AP) activities present in high-speed sediments from 
      spent culture media. However, adjusting the levels of all amino acids to match 
      those of the ECF produced the greatest stimulation of MV formation. Of the amino 
      acids that are notably enriched in ECF, glutamate (GLU), alanine (ALA), serine 
      (SER), asparagine (ASN), and taurine (TAU) individually enhanced MV production, 
      whereas proline (PRO), glycine (GLY), and aspartate (ASP) had essentially no 
      effect. The simple combination of ECF levels of ALA and GLU resulted in a 
      stimulation of MV formation equal to that observed when the eight aforementioned 
      amino acids were elevated to ECF levels. Other combinations of ASP and GLY, or of 
      TAU, SER, and ASN showed some stimulation, but at a lower level. Increasing the 
      amino acid concentrations, alone or in combination, also increased the levels of 
      cellular AP, and to a lesser extent cellular protein. While increases in cellular 
      AP were generally correlated with increased formation of AP-rich MV, this was not 
      uniformly true. These results indicate that in addition to hormones and growth 
      factors, nutritional factors such as the levels of amino acids are also critical 
      for normal phenotypic expression, growth, and matrix formation by epiphyseal 
      chondrocytes.
FAU - Ishikawa, Y
AU  - Ishikawa Y
FAU - Chin, J E
AU  - Chin JE
FAU - Schalk, E M
AU  - Schalk EM
FAU - Wuthier, R E
AU  - Wuthier RE
LA  - eng
GR  - AM 18983/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Amino Acids)
RN  - 0 (Culture Media)
SB  - IM
MH  - Amino Acids/*analysis
MH  - Animals
MH  - Cartilage/cytology
MH  - Cells, Cultured
MH  - Chickens
MH  - Culture Media
MH  - Epiphyses/*cytology/metabolism
MH  - Extracellular Matrix/metabolism/*ultrastructure
EDAT- 1986/03/01 00:00
MHDA- 1986/03/01 00:01
CRDT- 1986/03/01 00:00
PHST- 1986/03/01 00:00 [pubmed]
PHST- 1986/03/01 00:01 [medline]
PHST- 1986/03/01 00:00 [entrez]
AID - 10.1002/jcp.1041260310 [doi]
PST - ppublish
SO  - J Cell Physiol. 1986 Mar;126(3):399-406. doi: 10.1002/jcp.1041260310.