PMID- 3951253
OWN - NLM
STAT- MEDLINE
DCOM- 19860418
LR  - 20190824
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 10
IP  - 3
DP  - 1986
TI  - Identification of L3 leukemia and Burkitt's lymphoma cells by flow cytometric 
      quantitation of nuclear and cellular RNA and DNA content.
PG  - 303-12
AB  - Separate quantitations of nucleic acids of isolated nuclei and cells of L3 
      leukemia/Burkitt's lymphoma cell populations of peripheral blood (PB), bone 
      marrow (BM) and lymph node (LN) cell suspensions of 17 patients were performed by 
      acridine orange (AO) flow cytometry (FCM). The cell populations were analysed 
      with respect to cell cycle characteristics and DNA/RNA distribution histograms of 
      cells in various compartments of the cell cycle using mean value, coefficient of 
      variation (CV), third moment about the mean as a measure of skewness (MOM3). The 
      correlations between numbers of L3 blasts detected by microscopy and aneuploid 
      cells quantified by FCM in 10 patients with aneuploid disease were r = 0.75 and r 
      = 0.85 for BM and PB cell populations, respectively. Content of both nuclear and 
      cellular RNA was 3-4 times higher in L3 cells than in normal donor lymphocytes. 
      Percentages of cells in SG2M phase of L3 populations were significantly different 
      from control populations in BM (p less than 0.01) and PB (p less than 0.0001). 
      All samples with L3 blasts had abnormal CV of the DNA, and abnormal CV and MOM3 
      of the RNA peaks of G0/1 cell histograms. All samples with no blasts by 
      morphology had normal DNA and RNA patterns. AO FCM correctly classified all 
      specimens as involved or not involved with disease in accordance with the 
      cytomorphological diagnoses. Combining AO FCM with prior cell separation 
      increased the sensitivity of the method to detect abnormal cells to 0.02%, or 0.5 
      L3 cells per microliter of blood.
FAU - Walle, A J
AU  - Walle AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Burkitt Lymphoma/*analysis
MH  - Cell Cycle
MH  - Cell Nucleus/*analysis
MH  - DNA, Neoplasm/*analysis
MH  - *Flow Cytometry
MH  - Humans
MH  - Leukemia/*blood
MH  - RNA, Neoplasm/*analysis
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
AID - 10.1016/0145-2126(86)90027-5 [doi]
PST - ppublish
SO  - Leuk Res. 1986;10(3):303-12. doi: 10.1016/0145-2126(86)90027-5.