PMID- 3959145
OWN - NLM
STAT- MEDLINE
DCOM- 19860520
LR  - 20190711
IS  - 0022-5282 (Print)
IS  - 0022-5282 (Linking)
VI  - 26
IP  - 4
DP  - 1986 Apr
TI  - The acridine orange fluorochrome microassay: a new technique for quantitation of 
      neutrophil function in burned patients.
PG  - 389-92
AB  - A new assay for determination of neutrophil bacterial killing and phagocytosis is 
      presented. The acridine orange (AO) fluorochrome microassay is a simple, reliable 
      technique for assessing polymorphonuclear (PMN) function. It requires small 
      amounts of blood and provides a rapid and reproducible quantitation of neutrophil 
      activity. Using this technique, bacterial killing and phagocytosis were assessed 
      in a group of five severely burned patients admitted to the Medical College of 
      Virginia Burn Unit. All patients studied demonstrated a significant decrease in 
      bacterial killing at some point during their clinical course. The AO assay was 
      found to be a reliable and effective means of quantitating PMN bacterial 
      phagocytosis and killing in this group of burned patients.
FAU - Carpenter, A B
AU  - Carpenter AB
FAU - Boykin, J V Jr
AU  - Boykin JV Jr
FAU - Crute, S L
AU  - Crute SL
FAU - Haynes, B W
AU  - Haynes BW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Trauma
JT  - The Journal of trauma
JID - 0376373
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Adult
MH  - Burns/complications/*immunology
MH  - Humans
MH  - Middle Aged
MH  - Neutrophils/*physiology
MH  - Phagocyte Bactericidal Dysfunction/etiology
MH  - Phagocytosis
MH  - Time Factors
EDAT- 1986/04/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1986/04/01 00:00
PHST- 1986/04/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1986/04/01 00:00 [entrez]
AID - 10.1097/00005373-198604000-00014 [doi]
PST - ppublish
SO  - J Trauma. 1986 Apr;26(4):389-92. doi: 10.1097/00005373-198604000-00014.