PMID- 3960397
OWN - NLM
STAT- MEDLINE
DCOM- 19860429
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 64
IP  - 2
DP  - 1986 Feb 28
TI  - Evidence for the inhibitory subunit of adenylate cyclase (Ni) in nervous and 
      heart tissue of Aplysia.
PG  - 145-50
AB  - Cyclic adenosine 3',5'-monophosphate (cAMP) plays a critical role in modulating a 
      variety of neuronal responses in Aplysia californica. Previous studies have 
      focused on the neurotransmitter activation of adenylate cyclase, which presumably 
      occurs via the guanosine 5'-triphosphate (GTP)-regulated excitatory subunit (Ns). 
      While adenylate cyclase has also been shown to be regulated by inhibitory 
      neurotransmitters, coupled through the inhibitory GTP-regulated coupling protein 
      Ni in some systems, the effects of Ni-mediated adenylate cyclase inhibition on 
      neuronal processing in Aplysia have not previously been reported. In the present 
      study Ni is detected in Aplysia by both protein chemistry and enzymatic activity. 
      A 40 kdalton substrate for the enzymatic activity of Bordetella pertussis toxin 
      is observed. Incubation of Aplysia nervous tissue homogenates with pertussis 
      toxin (IAP) and 32P-nicotinamide-adenine dinucleotide labels a single protein, 
      assessed by polyacrylamide gel electrophoresis and autoradiography. Furthermore, 
      crude membrane suspensions of this tissue demonstrate biphasic adenylate cyclase 
      activity in response to increasing concentrations of GTP, showing Ni and Ns 
      functional activities. These findings provide evidence that Ni is present in 
      Aplysia tissue. Ni may serve as an important site for the regulation of cAMP 
      synthesis and neuronal plasticity.
FAU - Critz, S D
AU  - Critz SD
FAU - Harper, J F
AU  - Harper JF
FAU - Byrne, J H
AU  - Byrne JH
LA  - eng
GR  - DE-06511/DE/NIDCR NIH HHS/United States
GR  - EY-07024/EY/NEI NIH HHS/United States
GR  - NS-19895/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Adenylate Cyclase Toxin)
RN  - 0 (Adenylyl Cyclase Inhibitors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylate Cyclase Toxin
MH  - *Adenylyl Cyclase Inhibitors
MH  - Adenylyl Cyclases/metabolism
MH  - Animals
MH  - Aplysia/*analysis
MH  - Autoradiography
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Ganglia/analysis
MH  - Guanosine Triphosphate/pharmacology
MH  - Myocardium/analysis
MH  - Nerve Tissue Proteins/*analysis
MH  - Pertussis Toxin
MH  - Virulence Factors, Bordetella/metabolism
EDAT- 1986/02/28 00:00
MHDA- 1986/02/28 00:01
CRDT- 1986/02/28 00:00
PHST- 1986/02/28 00:00 [pubmed]
PHST- 1986/02/28 00:01 [medline]
PHST- 1986/02/28 00:00 [entrez]
AID - 0304-3940(86)90090-X [pii]
AID - 10.1016/0304-3940(86)90090-x [doi]
PST - ppublish
SO  - Neurosci Lett. 1986 Feb 28;64(2):145-50. doi: 10.1016/0304-3940(86)90090-x.