PMID- 3961459
OWN - NLM
STAT- MEDLINE
DCOM- 19860507
LR  - 20140728
IS  - 0036-7672 (Print)
IS  - 0036-7672 (Linking)
VI  - 116
IP  - 14
DP  - 1986 Apr 5
TI  - [Prospective evaluation of coagglutination and latex agglutination in the 
      diagnosis of bacterial meningitis in childhood].
PG  - 431-40
AB  - Two simple and low-priced kits to detect bacterial antigens in cerebrospinal 
      fluid (CSF) specimens from patients with suspected meningitis have recently 
      become available. Methods employed by these kits are staphylococcal 
      coagglutination (COA) and latex agglutination (LA). The COA "Phadebact CSF-kit" 
      (Pharmacia) detects Haemophilus influenzae type b (HIb), Neisseria meningitidis 
      (NM) groups A, B, C, Y and W 135 and Streptococcus pneumoniae (SP), whereas the 
      LA "Slidex Meningite-kit" (BioMerieux) includes HIb, NM A, NM C and SP. These two 
      diagnostic tests were compared with the standard methods for analyzing CSF 
      specimens from children with suspected meningitis. A total of 336 CSF specimens 
      were tested. Forty-three were from children with bacterial meningitis due to HIb, 
      NM or SP obtained before antimicrobial therapy. Thirty-four of them (79%) were 
      correctly detected by COA, 23 (54%) by LA, and 42 (97%) by microscopic analysis 
      of stained smears. There were 65 CSF samples of such meningitis cases obtained 
      after initiation of antimicrobial therapy: COA detected 26 (40%), LA 17 (26%) and 
      staining 26 (40%). With culture-positive CSF specimens from patients before 
      therapy, the best sensitivities were obtained with LA for SP (100%) and COA for 
      HIb (87%), whereas the results for NM antigen detection were only 43% with COA 
      and 0% with LA (no reagent against NM B). Cross-reactions with other bacterial 
      antigens were frequent with COA (26%) and rare with LA (2%), resulting in 
      false-positive findings in 6 of the 66 positive tests with COA (9%) but in none 
      of the 40 positive tests with LA (0%). One of the 48 specimens from aseptic 
      meningitis cases was false-positive for HIb by COA, whereas all 172 normal 
      specimens were negative with both methods. With 5 culture-negative CSF specimens 
      from patients with unequivocal purulent meningitis COA detected bacterial antigen 
      in 5 and LA in 2. CONCLUSIONS: The two kits evaluated cannot replace standard 
      methods for analyzing CSF specimens, in view of insufficient sensitivity (mainly 
      LA) or frequent cross-reactions and false-positive values (COA). However, due to 
      their simplicity, rapidity, and possible identification of bacterial pathogens 
      after initiation of antimicrobial therapy, these kits are recommended as an 
      useful addition to standard methods.
FAU - Kobelt, R
AU  - Kobelt R
FAU - Schaad, U B
AU  - Schaad UB
LA  - ger
PT  - English Abstract
PT  - Journal Article
TT  - Prospektive Evaluation von Coagglutination und Latex-Agglutination in der 
      Diagnostik der bakteriellen Meningitiden im Kindesalter.
PL  - Switzerland
TA  - Schweiz Med Wochenschr
JT  - Schweizerische medizinische Wochenschrift
JID - 0404401
RN  - 0 (Antigens, Bacterial)
SB  - IM
MH  - Adolescent
MH  - *Agglutination Tests
MH  - Antigens, Bacterial/immunology
MH  - Bacterial Infections/cerebrospinal fluid/*immunology/microbiology
MH  - Child
MH  - Child, Preschool
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Latex Fixation Tests
MH  - Meningitis/cerebrospinal fluid/*immunology/microbiology
MH  - Prospective Studies
EDAT- 1986/04/05 00:00
MHDA- 1986/04/05 00:01
CRDT- 1986/04/05 00:00
PHST- 1986/04/05 00:00 [pubmed]
PHST- 1986/04/05 00:01 [medline]
PHST- 1986/04/05 00:00 [entrez]
PST - ppublish
SO  - Schweiz Med Wochenschr. 1986 Apr 5;116(14):431-40.