PMID- 3963819
OWN - NLM
STAT- MEDLINE
DCOM- 19860512
LR  - 20231213
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 246
IP  - 1
DP  - 1986 Apr
TI  - Race:cultivar-specific induction of enzymes related to phytoalexin biosynthesis 
      in soybean roots following infection with Phytophthora megasperma f. sp. 
      glycinea.
PG  - 149-54
AB  - Primary roots of soybean [Glycine max (L.), cv Harosoy 63] seedlings were 
      inoculated with zoospores from either race 1 (incompatible, host resistant) or 
      race 3 (compatible, host susceptible) of Phytophthora megasperma f. sp. glycinea 
      (Pmg) and the activities of phenylalanine ammonia-lyase (PAL), chalcone synthase 
      (CHS), isoflavone synthase, and dihydroxypterocarpan 6a-hydroxylase related to 
      phytoalexin (glyceollin) biosynthesis, and of glucose-6-phosphate dehydrogenase 
      (Glc-6-PDH) and glutamate dehydrogenase (Glu-DH) were determined at various times 
      after inoculation. About 2-4 h after inoculation with race 1, the activities of 
      PAL, CHS, and pterocarpan 6a-hydroxylase were higher than after inoculation with 
      race 3 and increased considerably thereafter. In contrast, activities of these 
      enzymes in the compatible interaction were equal to or only slightly higher than 
      in the controls over the entire infection period investigated (2-8 h). Isoflavone 
      synthase did not increase until 7 h after inoculation with race 1. There were no 
      significant differences in activities for Glc-6-PDH and Glu-DH between inoculated 
      roots and controls. The results show that infection of soybean roots with 
      zoospores of Pmg race 1 causes a race:cultivar-specific early induction of 
      enzymes involved in glyceollin synthesis, whereas such an induction does not 
      occur with zoospores of race 3. These findings are in agreement with the 
      race:cultivar-specific accumulation of glyceollin in soybean roots reported 
      previously [M. G. Hahn, A. Bonhoff, and H. Grisebach (1985) Plant Physiol. 77, 
      591-601].
FAU - Bonhoff, A
AU  - Bonhoff A
FAU - Loyal, R
AU  - Loyal R
FAU - Ebel, J
AU  - Ebel J
FAU - Grisebach, H
AU  - Grisebach H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Plant Extracts)
RN  - 0 (Sesquiterpenes)
RN  - 0 (Soybean Proteins)
RN  - 0 (Terpenes)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 1.13.- (Oxygenases)
RN  - EC 1.14.13.- (cytochrome P450 CYP93A1 (Glycine max))
RN  - EC 1.14.13.- (isoflavone synthase)
RN  - EC 1.4.1.2 (Glutamate Dehydrogenase)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.74 (flavanone synthetase)
RN  - EC 4.3.1.24 (Phenylalanine Ammonia-Lyase)
RN  - 0 (Phytoalexins)
SB  - IM
MH  - Acyltransferases/metabolism
MH  - *Chytridiomycota
MH  - *Cytochrome P-450 Enzyme System
MH  - Enzyme Induction
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Glutamate Dehydrogenase/metabolism
MH  - Mixed Function Oxygenases/metabolism
MH  - Oxygenases/metabolism
MH  - Phenylalanine Ammonia-Lyase/metabolism
MH  - *Phytophthora
MH  - *Plant Diseases
MH  - Plant Extracts/*biosynthesis
MH  - Sesquiterpenes
MH  - Soybean Proteins
MH  - Glycine max/enzymology/*metabolism
MH  - Species Specificity
MH  - Terpenes
MH  - Phytoalexins
EDAT- 1986/04/01 00:00
MHDA- 1986/04/01 00:01
CRDT- 1986/04/01 00:00
PHST- 1986/04/01 00:00 [pubmed]
PHST- 1986/04/01 00:01 [medline]
PHST- 1986/04/01 00:00 [entrez]
AID - 0003-9861(86)90458-3 [pii]
AID - 10.1016/0003-9861(86)90458-3 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1986 Apr;246(1):149-54. doi: 10.1016/0003-9861(86)90458-3.