PMID- 3964813
OWN - NLM
STAT- MEDLINE
DCOM- 19850122
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 134
IP  - 1
DP  - 1985 Jan
TI  - Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and 
      systemic lupus erythematosus demonstrate similar nucleic acid binding 
      characteristics.
PG  - 180-6
AB  - Hybridoma anti-DNA antibodies have been generated from the fusion of the GM 4672 
      lymphoblastoid line with peripheral blood lymphocytes from four normal subjects, 
      nine patients with rheumatoid arthritis (RA), and 13 patients with systemic lupus 
      erythematosus (SLE). A total of 441 hybridoma clones were obtained, of which 37 
      secreted anti-DNA autoantibodies. The nucleic acid binding characteristics of the 
      anti-DNA antibodies produced by two hybridomas from normal subjects, nine 
      hybridomas from RA patients, and 18 hybridomas from SLE patients are reported. 
      The hybridoma anti-DNA antibodies from all three groups showed similar 
      antigen-binding characteristics for denatured DNA (dDNA), native DNA (nDNA), 
      poly(I), poly(dT), and cardiolipin, by both direct binding and competitive 
      binding analyses. One difference noted between normal-derived anti-DNA antibodies 
      and autoimmune-derived antibodies was the inability of the former to react with 
      z-DNA. However, this requires further substantiation with larger numbers of 
      normal-derived clones. The broad overlap of reactivity to nucleic acid antigens 
      among individual anti-DNA autoantibodies found in two clinically different 
      autoimmune diseases, namely RA and SLE, suggests that the pathogenicity of 
      anti-DNA autoantibodies may bear no relationship to their nucleic acid 
      antigen-binding characteristics.
FAU - Rauch, J
AU  - Rauch J
FAU - Massicotte, H
AU  - Massicotte H
FAU - Tannenbaum, H
AU  - Tannenbaum H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Autoantibodies)
RN  - 0 (Cardiolipins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Antigen-Antibody Complex
MH  - Arthritis, Rheumatoid/*immunology
MH  - Autoantibodies/*analysis
MH  - Cardiolipins/analysis
MH  - Cell Line
MH  - Clone Cells
MH  - DNA/analysis/*immunology
MH  - Female
MH  - Humans
MH  - Hybridomas
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Lymphocytes/*immunology
MH  - Male
MH  - Middle Aged
MH  - Reference Values
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1985 Jan;134(1):180-6.