PMID- 3970731
OWN - NLM
STAT- MEDLINE
DCOM- 19850321
LR  - 20190718
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 28
IP  - 2
DP  - 1985 Feb
TI  - The binding of antihistone antibodies to Crithidia luciliae kinetoplasts is 
      growth cycle-dependent.
PG  - 163-8
AB  - The Crithidia luciliae immunofluorescence (CLIF) assay is widely used to test for 
      native DNA (nDNA) antibodies in the diagnosis and management of systemic lupus 
      erythematosus. However, sera from patients with drug-induced lupus erythematosus 
      or rheumatoid arthritis, which should not contain nDNA antibodies, occasionally 
      react with the CL kinetoplast. We examined 36 sera from patients with systemic 
      lupus erythematosus, rheumatoid arthritis, Sjogren's syndrome, and drug-induced 
      lupus erythematosus, who had positive CLIF tests. All 36 sera were also 
      antinuclear antibody-positive with homogeneous and/or peripheral staining 
      patterns on mouse kidney substrates. After hydrochloric acid extraction of the CL 
      smears to remove histone and other nuclear protein antigens, 14 of the 36 sera no 
      longer produced a positive result on the CLIF test. Ten of these 14 sera again 
      gave a positive CLIF result after the hydrochloric acid-extracted Crithidia 
      substrate had been reconstituted with purified histone. These studies 
      demonstrated that kinetoplast binding was due to antihistone antibodies in at 
      least 10 of 36 initially CLIF-positive sera. Antihistone antibodies were then 
      purified with a histone-affinity column, and these purified antibodies were 
      reactive with CL kinetoplasts. Thus, the CLIF test is not specific for nDNA 
      antibodies. Additional studies using CL from different days of culture indicated 
      that histone antigen expression in the CL kinetoplast was a function of the life 
      cycle of this organism and is most readily detected 2 days after initiation of 
      culture.
FAU - Deng, J S
AU  - Deng JS
FAU - Sontheimer, R D
AU  - Sontheimer RD
FAU - Lipscomb, M F
AU  - Lipscomb MF
FAU - Gilliam, J N
AU  - Gilliam JN
LA  - eng
GR  - AI17363/AI/NIAID NIH HHS/United States
GR  - AM00730/AM/NIADDK NIH HHS/United States
GR  - AM19101/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Autoantibodies)
RN  - 0 (Histones)
SB  - IM
MH  - Animals
MH  - Arthritis, Rheumatoid/immunology
MH  - Autoantibodies/*immunology
MH  - *Binding Sites, Antibody
MH  - Crithidia/growth & development/*immunology
MH  - Histones/*immunology
MH  - Humans
MH  - Kidney/immunology
MH  - Kinetics
MH  - Lupus Erythematosus, Systemic/immunology
MH  - Mice/immunology
EDAT- 1985/02/01 00:00
MHDA- 1985/02/01 00:01
CRDT- 1985/02/01 00:00
PHST- 1985/02/01 00:00 [pubmed]
PHST- 1985/02/01 00:01 [medline]
PHST- 1985/02/01 00:00 [entrez]
AID - 10.1002/art.1780280210 [doi]
PST - ppublish
SO  - Arthritis Rheum. 1985 Feb;28(2):163-8. doi: 10.1002/art.1780280210.