PMID- 3972157
OWN - NLM
STAT- MEDLINE
DCOM- 19850322
LR  - 20190721
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 107
IP  - 2
DP  - 1985 Feb
TI  - Cell cycles in the male accessory glands of mealworm pupae.
PG  - 314-24
AB  - During the pupal stage of Tenebrio molitor, the accessory reproductive glands of 
      males grow by cell division. Within the secretory epithelium of the bean-shaped 
      accessory glands (BAGs), cell numbers triple. In the tubular accessory glands 
      (TAGs), the increase is 14-fold. There are two mitotic maxima in each gland. The 
      first maximum occurs at 1-2 days while the second is at 4-5 days. The second 
      maximum coincides with the major ecdysteroid peak described by Delbecque et al. 
      [Dev. Biol. 64, 11-30 (1978)]. Nuclei were isolated from TAGs during the pupal 
      mitotic bouts and during mitotic inactivity in the adult. After Feulgen or 
      propidium iodide staining, the DNA content of these nuclear populations was 
      measured by absorption cytophotometry or by fluorescence flow cytometry, 
      respectively. The proportion of cells in each phase of the cycle was calculated 
      using an iterative model. After mitoses have ended in the late pupa, the cells 
      were arrested in G2. [3H]Thymidine was injected into 1- and 4-day pupae to 
      pulse-label cells of the TAGs. After allowing various periods from 4 to 60 hr for 
      cells to progress through G2 to reach mitosis, fractions of labelled mitoses were 
      determined by autoradiography. From the combined cytometric and autoradiographic 
      data, the duration of each phase of the cell cycle was calculated assuming the 
      population was in exponential growth. Cell cycles in 4-day pupal TAGs take 48 hr. 
      G1, S, G2, and, M lasted 13, 14, 17, and 4 hr, respectively.
FAU - Happ, G M
AU  - Happ GM
FAU - MacLeod, B J
AU  - MacLeod BJ
FAU - Szopa, T M
AU  - Szopa TM
FAU - Bricker, C S
AU  - Bricker CS
FAU - Lowell, T C
AU  - Lowell TC
FAU - Sankel, J H
AU  - Sankel JH
FAU - Yuncker, C
AU  - Yuncker C
LA  - eng
GR  - A1-15662/PHS HHS/United States
GR  - GM21640/GM/NIGMS NIH HHS/United States
GR  - RR-01510/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
SB  - IM
MH  - Animals
MH  - *Cell Cycle
MH  - *Cell Differentiation
MH  - Male
MH  - Mitosis
MH  - Pupa/anatomy & histology
MH  - Tenebrio/*cytology
EDAT- 1985/02/01 00:00
MHDA- 1985/02/01 00:01
CRDT- 1985/02/01 00:00
PHST- 1985/02/01 00:00 [pubmed]
PHST- 1985/02/01 00:01 [medline]
PHST- 1985/02/01 00:00 [entrez]
AID - 0012-1606(85)90314-8 [pii]
AID - 10.1016/0012-1606(85)90314-8 [doi]
PST - ppublish
SO  - Dev Biol. 1985 Feb;107(2):314-24. doi: 10.1016/0012-1606(85)90314-8.