PMID- 3981059
OWN - NLM
STAT- MEDLINE
DCOM- 19850507
LR  - 20180206
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 105
IP  - 4
DP  - 1985 Apr
TI  - Renal secretion of phenolsulfonphthalein: analysis of its vectorial transport in 
      normal and mutant analbuminemic rats.
PG  - 484-8
AB  - Transtubular transport of many organic anions, such as p-aminohippuric acid and 
      phenolsulfonphthalein (PSP), from plasma into urine is an important renal 
      function. Most of these nephrophilic ligands strongly bind to albumin in the 
      circulation. To investigate a possible function of plasma albumin in vectorial 
      transport of these organic anions, plasma clearance and urinary excretion of PSP, 
      on one hand, and its interaction with serum proteins, on the other, were studied 
      in normal and mutant Nagase analbuminemic rats (NAR). Intravenously administered 
      PSP rapidly disappeared from the circulation, followed by its urinary excretion 
      in both NAR and normal rats. However, its plasma clearance was significantly 
      larger in NAR (53.9 ml/min/kg body weight) than in normal animals (4.7 ml/min/kg 
      body weight). Gel exclusion Sephadex G-100 chromatography and ultrafiltration 
      analysis revealed that the PSP binding capacity of serum proteins was 
      considerably lower in NAR than in normal rats; 32.0% and 12.5% of the ligand 
      bound to NAR serum protein and 94.4% and 84.2% to normal rat serum protein 
      (predominantly albumin) at 0.1 and 0.5 mmol/L ligand concentrations, 
      respectively. Despite the greater PSP clearance in NAR, its urinary excretion was 
      lower in NAR than in the normal animals; 20.9% +/- 2.5% and 46.0% +/- 12.6% of 
      the administered dose appeared in NAR and normal rat urine, respectively, within 
      3 hours of administration. Injection of PSP with equimolar albumin resulted in a 
      decrease in plasma clearance and an increase in urinary excretion of PSP in NAR; 
      more than 31.4% +/- 3.3% of the injected dose appeared in the urine within 3 
      hours of administration.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Inoue, M
AU  - Inoue M
FAU - Koyama, H
AU  - Koyama H
FAU - Nagase, S
AU  - Nagase S
FAU - Morino, Y
AU  - Morino Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Albumins)
RN  - 0 (Blood Proteins)
RN  - 0 (Phenolphthaleins)
RN  - I6G9Y0J1OJ (Phenolsulfonphthalein)
SB  - IM
MH  - Albumins/metabolism/*pharmacology
MH  - Animals
MH  - Blood Proteins/metabolism
MH  - Chromatography, Gel
MH  - Injections, Intravenous
MH  - Kidney/drug effects/*metabolism
MH  - Male
MH  - Phenolphthaleins/*metabolism
MH  - Phenolsulfonphthalein/blood/*metabolism/urine
MH  - Protein Binding
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Spectrophotometry
EDAT- 1985/04/01 00:00
MHDA- 1985/04/01 00:01
CRDT- 1985/04/01 00:00
PHST- 1985/04/01 00:00 [pubmed]
PHST- 1985/04/01 00:01 [medline]
PHST- 1985/04/01 00:00 [entrez]
AID - 0022-2143(85)90173-8 [pii]
PST - ppublish
SO  - J Lab Clin Med. 1985 Apr;105(4):484-8.